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Journal / Biochemistry / 初代ヒト気道上皮細胞の気道表面液 pH…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
初代ヒト気道上皮細胞の気道表面液 pH をリアルタイムで半自動で蛍光測定
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
Journal (Biochemistry)
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
 
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初代ヒト気道上皮細胞の気道表面液 pH をリアルタイムで半自動で蛍光測定

Article DOI: 10.3791/59815-v • 10:18 min • June 13th, 2019
June 13th, 2019 •
1, 2,3, 2, 2,4, 1,2, 2,3, 1
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co

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我々は、プレートリーダーを用いて、薄膜状態下で気道表面液 pH の動的測定を行うためのプロトコールを提示する。

Tags

生化学、問題148、気道表面液、酸塩基平衡、pH、プレートリーダー、空気液界面、気道上皮、嚢胞性線維症
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