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JoVE Journal
Immunology and Infection

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Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
 

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Article DOI: 10.3791/59819-v
July 16th, 2019

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Summary July 16th, 2019

Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.

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