Journal
/
Immunology and Infection
/
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

DOI:
10.3791/59819-v

•

09:11 min

•

July 16, 2019

•

, , ,
1The Kennedy Institute of Rheumatology,The University of Oxford

Chapters

  • 00:04Title
  • 01:03Listeria monocytogenes (LM) Infection and Brefeldin A (BFA) Treatment
  • 02:02Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
  • 04:41Immunofluorescent Staining and Imaging
  • 06:44Results: IFNγ Production
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.

Tags

Keywords: In SituInterferon GammaListeria MonocytogenesMouse SpleenCytokine VisualizationMicroenvironmentLM OVA CultureIntravenous InjectionBFAEuthanizationSpleen DissectionFixative Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59819?article_type=v

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
check_url/59819?article_type=v

Following Cell-fate in E. coli After Infection by Phage Lambda
check_url/59819?article_type=v

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
check_url/59819?article_type=v

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
check_url/59819?article_type=v

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics
check_url/59819?article_type=v

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
check_url/59819?article_type=v

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
check_url/59819?article_type=v

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
check_url/59819?article_type=v

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
check_url/59819?article_type=v

Listeria monocytogenes Infection of the Brain

Read Article