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JoVE Journal
Immunology and Infection

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Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
 

Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

Article DOI: 10.3791/59821-v
September 26th, 2019

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Summary September 26th, 2019

The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.

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