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Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
DOI:

10:20 min

August 09, 2019

Chapters

  • 00:04Title
  • 00:35Sample Stage, Photosynthetic Instrument, and Light Source Setup
  • 01:47Simultaneous Measurement Leaf Reflectance and Chlorophyll Fluorescence Analysis Setup
  • 02:49Actinic Light Irradiance Intensity Measurement
  • 04:23Simultaneous Measurement Leaf Reflectance and Chlorophyll Fluorescence Analysis
  • 06:41Photosynthetic Parameter Calculation
  • 08:26Representative Changes in Photosynthetic Parameters in Wild-Type Columbia and npq1 Mutant Arabidopsis Plants
  • 09:36Conclusion

Summary

Automatic Translation

We describe a new technical approach to study photosynthetic responses in higher plants involving simultaneous measurements of chlorophyll a fluorescence and leaf reflectance using a PAM and a spectral radiometer for the detection of signals from the same leaf area in Arabidopsis.

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