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Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
 

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Article doi: 10.3791/59843
July 18th, 2019

Summary July 18th, 2019

In this study, we describe a detailed protocol for inducing liver vesicular steatosis in differentiated HepaRG cells with the fatty acid salt sodium oleate and employ methods for detection and quantification of lipid accumulation, including coherent anti-Stokes Raman scattering (CARS) microscopy, cytofluorimetric analysis, Oil red O staining, and qPCR. 

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