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JoVE Journal
Immunology and Infection

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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
 

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Article doi: 10.3791/59853
June 24th, 2019

Summary June 24th, 2019

We describe a method to visualize GFP-labeled γδ IELs using intravital imaging of murine small intestine by inverted spinning disk confocal microscopy. This technique enables the tracking of live cells within the mucosa for up to 4 h and can be used to investigate a variety of intestinal immune-epithelial interactions.  

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