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Antibody-Free Assay for RNA Methyltransferase Activity Analysis
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JoVE Journal Genetics
Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

DOI:
10.3791/59856-v

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08:31 min

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July 09, 2019

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,
1Department of Molecular Biosciences,University of Texas at Austin

Chapters

  • 00:04Title
  • 00:40In Vitro RNA Methyltransferase Assay
  • 06:05Results: Analysis of RNA Methyltransferase Activity
  • 07:39Conclusion

Summary

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Here, an antibody-free in vitro assay for direct analysis of methyltransferase activity on synthetic or in vitro transcribed RNA is described.

Tags

Keywords: RNA MethyltransferaseAntibody-free AssayLiquid Scintillation CountingUrea Denaturing Polyacrylamide GelRNA MethylationRNA Modifications

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