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JoVE Journal Biochemistry
Vaskemiddel-assisteret rekonstitution af rekombinant Drosophila Atlastin til Liposomer til lipid-blanding assays
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Vaskemiddel-assisteret rekonstitution af rekombinant Drosophila Atlastin til Liposomer til lipid-blanding assays

Article DOI: 10.3791/59867-v • 08:43 min • July 3rd, 2019
July 3rd, 2019 •
1, 1
1Department of BioSciences, Rice University

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Biologisk membran fusion katalyseres af specialiserede fusions proteiner. Måling af proteiner fusogenic egenskaber kan opnås ved lipid blanding assays. Vi præsenterer en metode til rensning af rekombinant Drosophila atlastin, et protein, der medierer homotypisk fusion af ER, og som danner det til præformerede Liposomer og test for fusions kapacitet.

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Biokemi membran fusion rekonstitution fluorescerende resonans overførsel (FRET) lipid bilayer fosfolipid vesicle fjernelse af vaskemiddel transmembran domæne protein rensning lipid blanding assay atlastin rekombinant protein
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