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Cancer Research
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Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
 

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Article DOI: 10.3791/59880-v
July 27th, 2019

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Summary July 27th, 2019

This article outlines a simple PCR-based assay to monitor the activity of an active LINE-1 retrotransposon and to map de novo retrotranspositions in a given genome. Using the MCF7 cell line, we demonstrate herein how this method can be applied to detect activity of a LINE-1 located at 22q12.1.

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