Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
 

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Article DOI: 10.3791/59882-v 14:25 min September 21st, 2019
September 21st, 2019

Chapters

Summary

This work describes a robust and straightforward method to detect and quantify the endocannabinoid 2-arachidonoylglycerol (2-AG) in C. elegans. An analytical deuterated standard us prepared and used for the quantification of 2-AG by isotopic dilution and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS).

Tags

Keywords: 2-arachidonoylglycerol 2-AG Endocannabinoids Caenorhabditis Elegans Deuterated Standard Liquid Chromatography-mass Spectrometry Quantification Synthesis Cholesterol Trafficking
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter