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Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
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Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Article DOI: 10.3791/59884-v • 08:30 min • January 31st, 2020
January 31st, 2020 •
1, 2, 3, 1,3,4
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, 2Department of Urology, Medical University of South Carolina, 3Department of Urology, College of Medicine, University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center

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Summary
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We describe a method for preparation of the single freshly isolated detrusor smooth muscle cells from human urinary bladder specimens employing a two-step enzymatic procedure. The obtained viable DSM cells can be studied by various single cell techniques including the described amphotericin-B patch-clamp electrophysiology to reveal physiological and pharmacological properties.

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Freshly Isolated Human Detrusor Smooth Muscle Cells Characterization 9-Phenanthrol-Sensitive Cation Currents Urinary Bladder Protocol Single Smooth Muscle Cells Electrophysiological Examination Molecular Examination Control Normal Diseased Pharmacological Targets Ion Channels Patch-clamp Electrophysiology TRPM4 Channel Transient Receptor Potential Melastatin Four Channel Nonselective Cation Channel Dissection Enzymatic Treatment Steps Efficiency Familiarity Human Bladder Histology Isolated DSM Cells
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