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A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
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Chapters
- 00:04Title
- 01:04Mouse Infection
- 01:32Bioluminescence Monitoring of Mice Infected with BIRFLU
- 02:32Fluorescence Analysis in Mice Infected with BIRFLU
- 03:32Results: BIRFLU Characterization and Tracking
- 04:58Conclusion
Influenza A viruses (IAVs) are contagious respiratory pathogens that cause annual epidemics and occasional pandemics. Here, we describe a protocol to track viral infections in vivo using a novel recombinant luciferase and fluorescence-expressing bi-reporter IAV (BIRFLU). This approach provides researchers with an excellent tool to study IAV in vivo.
