Journal
/
Immunology and Infection
/
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

DOI:
10.3791/59905-v

•

05:57 min

•

November 23, 2019

•

, ,
1Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

Chapters

  • 00:04Title
  • 00:48Sample Preparation
  • 01:39Imaging
  • 03:34Deconvolution and Data Quantification
  • 04:36Results: Representative Visualization and Quantification of Cell Division Inhibition
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.

Tags

Keywords: Live-cell Fluorescence MicroscopySubcellular Protein LocalizationCell Morphology ChangesBacteriaSample PreparationMembrane Staining DyeAgarose SlabHigh-resolution Deconvolution Microscope100x Oil Immersion ObjectiveResolve 3D Imaging SoftwareZ-stacksSample Thickness

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59905?article_type=v

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
check_url/59905?article_type=v

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
check_url/59905?article_type=v

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
check_url/59905?article_type=v

Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
check_url/59905?article_type=v

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
check_url/59905?article_type=v

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
check_url/59905?article_type=v

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
check_url/59905?article_type=v

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
check_url/59905?article_type=v

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
check_url/59905?article_type=v

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Read Article