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Journal / Bioengineering / Human iPSC-Derived Cardiomyocyte Networks on…
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Bioengineering

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JoVE Journal Bioengineering
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
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Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

Article DOI: 10.3791/59906-v • 08:53 min • July 15th, 2019
July 15th, 2019 •
*1, *1, 1, 1, 1,2,3
1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Summary
Automatic Translation

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

Tags

Human IPSC-derived Cardiomyocyte Networks Multiwell Micro-electrode Arrays Action Potential Recordings Electroporation Compound Testing Multiwell MEA Coding Cell Plating Droplet Prevention Custom Graphical User Interface MATLAB Workflow Parameter Extraction Stem Cell Thawing Cell Suspension Substrate-coated Six-well Plate Cell Culture Incubator
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