Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / A Murine Cell Line Based Model of Chronic CDK9…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdeff) in Cancers
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Journal (Genetics)
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
Journal (Genetics)
Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
 

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdeff) in Cancers

Article DOI: 10.3791/59910-v • 10:49 min • September 26th, 2019
September 26th, 2019 •
1, 1, 1
1Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center (CCHMC)

Chapters

Summary
Automatic Translation

The protocol details an in vitro murine carcinoma model of non-genetic defective transcription elongation. Here, chronic inhibition of CDK9 is used to repress productive elongation of RNA Pol II along pro-inflammatory response genes to mimic and study the clinically observed TEdeff phenomenon, present in about 20% of all cancer types.

Tags

Murine Cell Line Chronic CDK9 Inhibition Non-genetic Transcriptional Elongation Defects TEdeff Cancer Research Pharmacological Model Transcriptional And Epigenetic Defects Immunotherapy Novel Uses For Existing Drugs Tumor-immune Interactions In Vitro And In Vivo Studies Human Carcinoma Lines T47D CAL51 Sublethal Dose Of Flavopiridol Cell Plating Density Culture Conditions Cytokine Stimulation Conditions PolyA-positive RNA Ribosomal RNA-depleted Samples Oligo DT Magnetic Beads
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter