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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
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JoVE Journal Environment
Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

DOI:
10.3791/59931-v

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08:00 min

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October 18, 2019

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, ,
1Brain and Mind Institute,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:42Media Preparation and Sample Collection
  • 02:48TSC Sandwich Plating
  • 04:05Sub-culturing of Sulfite-Reducing Colonies
  • 05:08DNA Extraction and PCR Genotyping
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

The objective of this protocol is to detect different Clostridium perfringens toxinotypes in locally purchased foods, particularly epsilon toxin producing strain types B and D, without the use of anaerobic chambers.

Tags

Keywords: Clostridium PerfringensToxinotypesEnrichmentDetectionRetail Food SamplesEpsilon ToxinModified Rapid Perfringens Medium (RPM)D-cycloserineTryptose Sulfite Cycloserine (TSC) AgarAnaerobic CultureSpore Selection

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