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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
 

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Article doi: 10.3791/59955
March 24th, 2020

Summary March 24th, 2020

This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

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