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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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JoVE Journal Genetics
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

DOI:
10.3791/59955-v

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11:30 min

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March 24, 2020

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, , ,
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Chapters

  • 00:04Introduction
  • 01:42Tissue Harvesting and Processing
  • 06:34Slide Preparation
  • 08:25Results: Evaluation of DNA Damage
  • 10:45Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

Tags

Keywords: Comet AssayDNA DamageGenotoxicityPre-clinical Drug DevelopmentSafety AssessmentEnvironmental BiomonitoringSample Handling

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