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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
 

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Article doi: 10.3791/59960
September 2nd, 2019 Usage Statistics

Summary September 2nd, 2019

In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).

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