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JoVE Journal
Immunology and Infection

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Isolation, Transfection, and Culture of Primary Human Monocytes
 

Isolation, Transfection, and Culture of Primary Human Monocytes

Article DOI: 10.3791/59967-v
December 16th, 2019

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Summary December 16th, 2019

Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.

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