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JoVE Journal
Genetics

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Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
 

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Article DOI: 10.3791/59971-v 12:22 min August 18th, 2019
August 18th, 2019

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Summary

Parasponia andersonii is a fast-growing tropical tree that belongs to the Cannabis family (Cannabaceae) and can form nitrogen-fixing root nodules in association with the rhizobium. Here, we describe a detailed protocol for reverse genetic analyses in P. andersonii based on Agrobacterium tumefaciens-mediated stable transformation and CRISPR/Cas9-based genome editing.

Tags

Keywords: Parasponia Andersonii Nitrogen-fixing Tropical Tree Cannabaceae Genome Editing Phenotyping Transgenic Mutant Lines Rhizobial Symbiosis Tissue Culture In Vitro Propagation Seed Germination Plant Transformation
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