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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

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07:59 min

October 04, 2019

DOI:

07:59 min
October 04, 2019

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Summary

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Here we present easy to use protocols for producing and purifying diterpenoid metabolites through the combinatorial expression of biosynthetic enzymes in Escherichia coli or Nicotiana benthamiana, followed by chromatographic product purification. The resulting metabolites are suitable for various studies including molecular structure characterization, enzyme functional studies, and bioactivity assays.

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