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Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
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Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

DOI:
10.3791/59995-v

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00:08 min

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August 27, 2019

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, , ,
1Department of Pharmacology,University of Vermont Larner College of Medicine, 2University of Vermont Cancer Center, 3Department of Molecular Physiology and Biophysics,University of Vermont Larner College of Medicine

Chapters

  • 00:04Title
  • 00:41Glass Coverslip Activation
  • 01:15Fluorescent Microbead Deposition and Hydrogel Casting
  • 02:38Hydrogel Activation and Extracellular Matrix Coating
  • 03:38Cell Plating and Micropipette Preparation
  • 05:51Micromanipulator Calibration and Durotaxis Assay
  • 07:01Results: Representative Durotactic Stimulation Responses and Protein Expression Analyses
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.

Tags

Keywords: Single CellDurotaxisMechanical MicroenvironmentCell SignalingCell MigrationHydrogelFluorescent Micro-beadsSulfo-SANPAHUV Activation

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