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Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
 

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Article DOI: 10.3791/59999-v
July 6th, 2019

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Summary July 6th, 2019

Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

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