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Optical Clearing and Imaging of Immunolabeled Kidney Tissue
 

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Article doi: 10.3791/60002
July 22nd, 2019

Summary July 22nd, 2019

The combination of antibody labeling, optical clearing, and advanced light microscopy allows three-dimensional analysis of complete structures or organs. Described here is a simple method to combine immunolabeling of thick kidney slices, optical clearing with ethyl cinnamate, and confocal imaging that enables visualization and quantification of three-dimensional kidney structures.

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