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Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

DOI:
10.3791/60008-v

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07:40 min

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June 29, 2020

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, , , , , , , , , , , , , , , ,
1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies,Institut Pasteur, 2Environment and Sustainability Institute,University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5Direction des Services Vétérinaires, 6Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, 7Laboratoire Central Vétérinaire de Bingerville,Laboratoire National d’Appui au Développement Agricole Bingerville, 8FAO Reference Centre for Rabies,Istituto Zooprofilattico Sperimentale delle Venezie, 9Swiss Tropical and Public Health Institute, 10University of Basel, 11Institut National de Recherche Biomédicale

Chapters

  • 00:04introduction
  • 00:54Sample Collection and Modified Rabies Rapid Immunochromatographic Diagnostic test (RIDT) Execution
  • 03:46RNA Extraction and Detection
  • 05:28Results: Representative RIDT Testing
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

We present a complete protocol for postmortem diagnosis of animal rabies under field conditions using a rapid immunochromatographic diagnostic test (RIDT), from brain biopsy sampling to final interpretation. We also describe further applications using the device for molecular analysis and viral genotyping.

Tags

Keywords: RabiesRapid Immunochromatographic TestPostmortem DiagnosisBrain Stem SampleResource-limited SettingsMolecular ApplicationDiagnostic ProcedureField ProtocolAfricaAsia

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