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JoVE Journal
Biochemistry

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Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
 

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Article DOI: 10.3791/60009-v
December 31st, 2019

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Summary December 31st, 2019

We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.

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