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Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
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Chapters
- 00:05Title
- 00:40Egg Collection
- 01:53Scanning Electron Microscopy (SEM) Preparation: Embryo Chemical Drying
- 04:18SEM Preparation: Eggshell Air-Drying
- 04:53SEM Preparation: Fungal Culture Critical Point Drying
- 07:12Results: Representative Scanning Electron Microscopic (SEM) Analysis of Painted Turtle Embryos and Eggshells
- 08:37Conclusion
Here, we present detailed processing protocols for imaging delicate tissue samples using scanning electron microscopy (SEM). Three different processing methods, namely, hexamethyl disilazana (HMDS) chemical drying, simple air drying, and critical point drying are described for preparing rigid eggshells, embryos at early developmental stages, and fungal cultures respectively.
