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Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

DOI:
10.3791/60032-v

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09:24 min

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September 18, 2019

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, , , , , , ,
1College of Pharmacy, Duksung Innovative Drug Center,Duksung Women’s University, 2Korea Drug Development Platform using Radio-isotope,Korea Institute of Radiological & Medical Sciences

Chapters

  • 00:04Title
  • 01:06Endothelial Colony Forming Cell (ECFC) and Mesenchymal Stem Cell (MSC) Suspension Preparation and Fluorescent Dye Labeling
  • 03:20ECFC, MSC, or ECFC+MSC Spheroid Generation
  • 04:12Neutralized Collagen Gel Spheroid Embedding and Spheroid Sprouting Quantification
  • 06:29Results: Representative Sprout Formation from ECFC-only, MSC-only, and ECFC+MSC Spheroids
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

Three-dimensional co-culture spheroid angiogenesis assay system is designed to mimic the physiologic angiogenesis. Co-culture spheroids are formed by two human vascular cell precursors, ECFCs and MSCs, and embedded in collagen gel. The new system is effective for evaluating angiogenic modulators, and provides more relevant information to the in vivo study.

Tags

Keywords: 3D Co-culture SpheroidAngiogenesisEndothelial Colony Forming Cells (ECFCs)Mesenchymal Stem Cells (MSCs)Vascular Progenitor CellsCell InteractionsSproutingTubular MaturationPersonalized TreatmentsAbnormal Angiogenesis-related DiseasesCancerDrug DevelopmentTrypsin-EDTASingle Cell SuspensionPKH67PKH26Fluorescent DyeSpheroid Generation

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