Name: Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion (Video) | JoVE Journal
Uploaded: 2019-09-12T14:30:00.000+00:00
Duration: 6 min 27 s
Description: Learn microbially induced calcite precipitation by immersion to improve sandy soil strength and stability. This method enables uniform calcium carbonate precipitation for enhanced mechanical testing of treated samples.

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5 minutes

This work was supported by the National Science Foundation Grant No. 1531382 and MarTREC.

NOTE: All relevant material used in the following procedures are non-hazardous. Personal protective equipment (safety glasses, gloves, lab coat, full length pants, closed-toe shoes) are still needed.
1. Preparation of bacteria solution
<ol>
	<li>Preparation of growth medium (NH4-YE medium) 
	NOTE: The components of growth media per liter of deionized water are: 20 g of yeast extract; 10 g of (NH4)2SO4; and 0.13 M Tris buffer (pH 9.0).
	<ol>
		<li>Autoclave ingredients separately.</li>
		<li>Dissolve 20 g of yeast extract, and 10 g of (NH4)2SO4 in 1 L of deionized water containing 0.13 M Tris buffer.</li>
		<li>Mix the components together using magnetic stirrer post-sterilization.</li>
	</ol>
	</li>
	<li>Propagation procedure of Sporosarcina pasteurii 
	NOTE: Use 50 mL centrifuge tubes in this experiment.
	<ol>
		<li>Thaw the frozen bacteria in a vial.</li>
		<li>Open the vial.</li>
		<li>Transfer 0.1 mL of bacteria suspension to a centrifuge tube with 10 mL of fresh growth medium. Mix well by hand (inoculation rate is 1:100). Repeat 5 more bacterial suspensions with growth medium. Prepare a control tube only with 10 of fresh growth medium inside. 
		NOTE: The cryoprotectant used in the freeze/drying procedure may inhibit growth in the primary tube. The lids of tubes were tightened loosely in order to maintain the aerobic condition.</li>
		<li>Incubate all tubes in a shaker at 200 rpm at 30 &#176;C for 48 to 72 hours. Stop the incubation if the growth medium becomes turbid after 48 h. Otherwise, extend the incubation to the maximum of 72 h.</li>
		<li>Centrifuge the tubes with bacteria and growth medium at 4,000 x g for 20 min.</li>
		<li>Remove the supernatant, replace with 25 mL of fresh growth medium, and mix well using a vortex machine.</li>
		<li>Repeat steps 1.2.3-1.2.6 twice to fully stimulate the activity of bacteria.</li>
		<li>Use the suspension from the tubes in step 1.2.7 to inoculate more tubes with 25 mL of growth medium to enhance the culture of bacteria (inoculation rate is 1:100).</li>
		<li>Incubate all tubes in a shaker at 200 rpm at 30 &#176;C for 48 hours.</li>
		<li>Centrifuge the tubes with bacteria and growth medium at 4,000 x g for 20 min.</li>
		<li>Remove the supernatant, replace with fresh growth medium, and mix well using a vortex machine.</li>
		<li>Adjust the bacteria concentration using fresh growth medium before the MICP experiments. Calculate the bacteria concentration by optical density of the suspension at 600 nm, which was measured using a spectrophotometer. The OD600 in this experiment was 0.6.</li>
	</ol>
	</li>
</ol>
2. Preparation of cementation media
NOTE: Cementation media is used to provide chemicals to induce the calcite precipitation during the MICP treatment. The urea-Ca2+ molar ratio is 1:1. The chemical components of cementation media is shown in Table 1. The following procedure is for 20 L of cementation media with 0.5 M Ca.
<ol>
	<li>Prepare 20 L of water in a plastic box.</li>
	<li>Dissolve 200 g of NH4Cl, 60 g of nutrient broth, 42.4 g of NaHCO3, 600 g of urea, and 1470 g of CaCl2&#8729;2H2O in the 20 L of distilled water. Mix well using stirring rod.</li>
</ol>
3. Preparation of molds
<ol>
	<li>Preparation of full contact flexible mold (FCFM) 
	NOTE: The full contact flexible mold is made of geotextile. The geotextile has a grab tensile strength of 1,689 N, a trapezoidal tear strength of 667 N, an apparent opening size of 0.15 mm, a water flow rate of 34 mm/s, a thickness of 1.51 mm, and a unit mass of 200 g/m2. The size of mold can be varied to prepare different sample sizes (for example, unconfined compression test sample or direct shear test sample).
	<ol>
		<li>As the FCFM consists of an annular part, a bottom, and a cover, cut the geotextile into constituent parts of FCFM.</li>
		<li>Sew the three parts of FCFM together as shown in Figure 1.</li>
	</ol>
	</li>
	<li>Preparation of rigid full contact mold (RFCM) for bio-bricks 
	NOTE: The rigid full contact mold consists of a flexible layer and a rigid holder. The flexible layer is made of the same geotextile as the FCFM. The rigid holder is made of a polypropylene perforated sheet with 6.35 mm diameter staggered holes distributed on the polypropylene perforated sheet and the clearance distance between adjacent holes is 9.53 mm. One mold consist of three chambers and the size of each chamber is 177.8 mm in length, 76.2 mm in width and 38.1 mm in height. The size of RFCM can be varied to prepare different sample size. The holes in the rigid holder allow cementation media flow through the flexible layer freely.
	<ol>
		<li>Prepare the polypropylene perforated sheet for constituent pieces of the rigid holder.</li>
		<li>Assemble the pieces of rigid holder using plastic screws and nuts.</li>
		<li>Prepare the constituent parts of the geotextile flexible layer. The flexible layer consists of a bottom and a cover.</li>
		<li>Enclose the bottom of the flexible layer in the rigid holder.</li>
		<li>Once the sand is added into the mold, place the cover of flexible layer and fix by sewing on the top of the sand sample as shown in Figure 2.</li>
	</ol>
	</li>
	<li>Preparation of hollow brick mold 
	NOTE: The hollow brick mold includes a rigid holder, a flexible layer, and cardboard tubes. The size of the cardboard tube is 60 mm x 140 mm x 60 mm. Three chambers are included in one mold and the size of each mold chamber is 177.8 mm in length, 76.2 mm in width and 38.1 mm in height in this procedure.
	<ol>
		<li>Prepare the polypropylene perforated sheet for the constituent pieces of the rigid holder.</li>
		<li>Drill holes on the bottom of the rigid holder piece. The size of the holes is 61 mm in diameter. The location of holes in each chamber is shown in Figure 3a.</li>
		<li>Assemble the pieces of the rigid holder using plastic screws and nuts.</li>
		<li>Assemble the cardboard tubes in the drilled holes on the bottom of the rigid holder.</li>
		<li>Prepare the constituent parts of the geotextile flexible layer. The flexible layer consists of a bottom and a cover. Holes are also needed on the flexible layer at the same location of cardboard tubes.</li>
		<li>Once the sand is added into the mold, place the cover of flexible layer and fix by sewing on the top of the sand sample as shown in Figure 3b.</li>
	</ol>
	</li>
</ol>
4. Preparation of the batch reactor
NOTE: The reactor shown in Figure 4 consists of a plastic box, cementation media, a sample supported shelf, and air pumps. The soil samples can fully immerse into the cementation media while the cementation media can freely diffuse into the soil samples by this method. The air pump in the reactor provides oxygen for bacteria. To determine the effects of different oxygen supply on MICP treatment catalyzed by Sporosarcina pasteurii, Li et al. 201718 conducted contrast tests under three different conditions: an aerated condition, an air restricted condition, and an open-air condition. They found that a well-oxygenated condition is essential to improve MICP processes catalyzed by aerobic bacteria.
<ol>
	<li>Connect the air pump with air supply using a plastic hose.</li>
	<li>Place the air pump in the plastic box.</li>
	<li>Pour cementation media into the plastic box.</li>
</ol>
5. Preparation of soil samples
<ol>
	<li>Preparation of MICP-treated soil sample 
	NOTE: Ottawa sand (99.7% quartz) is used in the experiments. The sand is uniform with a median particle size of 0.46 mm and no fines are included. It is classified as poorly graded sand based on the Unified Soil Classified System (USCS).
	<ol>
		<li>Add dry sand into molds by the air pluviation method (FCFM, RFCM, hollow brick mold) to reach a median dense condition (Dr in the range of approximately 42&#8211;55%, and dry density of sand in the range of 1.58&#8211;1.64 g/cm3). 
		NOTE: The weight of sand varies according to different kinds of molds: 145 &#177; 5 g sand for the UCS test sample, which is 38.6 mm in diameter and 76.2 mm in height.</li>
		<li>Place the cover on the top of samples and fix it by sewing.</li>
		<li>Pour the bacteria solution with a fixed optical density value through the permeable geotextile cover into the samples and make sure that they are saturated. 
		NOTE: The amount of bacteria solution varied according to different samples: 50 mL of bacteria solution for a UCS test sample, which is 38.6 mm in diameter and 76.2 mm in height.</li>
		<li>Place samples on the sample supported shelf as shown in Figure 5a.</li>
		<li>Immerse the entire shelf into the batch reactor filled with cementation media.</li>
		<li>Turn on the air supply and adjust the air output to keep 100% air saturation. Wait for 7 days of MICP reaction.</li>
		<li>Take out the samples from the reactor as shown in Figure 5b.</li>
		<li>Remove the samples by cutting the full contact flexible mold or demolding the rigid holder and then cutting the flexible layer.</li>
		<li>Wash the samples with water to remove the residual solution in the pore space.</li>
		<li>Place the samples in the 105 &#176;C oven for 48 h until their weights remain constant. The samples can be tested or treated additionally after the oven drying.</li>
	</ol>
	</li>
	<li>Preparation of fiber reinforced MICP-treated soil sample 
	NOTE: Synthetic fiber (see Table of Materials) and natural palm fiber as shown in Figure 6 are used in these procedures.
	<ol>
		<li>For the synthetic fiber, mix the proposed content of fibers and 900 g of dry sand in small increments by hand to obtain a uniform mixture. The fiber content in this experiment is fixed as 0.3% by weight of the dry sand.</li>
		<li>For the natural palm fiber, distribute 760 g of sand into four equal parts. Add these four parts of sand and three layers of fiber in RFCM at intervals.</li>
		<li>Repeat the same procedure as steps 5.1.2&#8212;5.1.10 to get the MICP-treated sample.</li>
	</ol>
	</li>
	<li>Preparation of cement-treated bricks with bio-surface treatment 
	NOTE: Portland cement (TYPE I/II) with specific gravity of 3.15 is used as the cementing agent for the cement-treated samples in this experiment. The early strength gain of this cement allowed the various curing times ranged from 7 to 21 days. The proportion of added cement in this procedure is 10% by weight of dry sand.
	<ol>
		<li>Mix 900 g of sand, 90 g of cement, and 200 mL of water to achieve a uniform mixture.</li>
		<li>Add the mixture to the rigid mold. The size of rigid mold is 177.8 mm in length, 76.2 mm in width and 38.1 mm in height.</li>
		<li>Cure for 7 days at a constant humidity of 100% and constant temperature of 25 &#176;C.</li>
		<li>Place samples in the 105 &#176;C oven for 48 hours until their weights remain constant.</li>
		<li>Repeat the same procedure as steps 5.1.3&#8212;5.1.8.</li>
		<li>Place samples in the 105 &#176;C oven for 48 hours until their weights remain constant. The samples can be tested or treated additionally after the oven drying.</li>
	</ol>
	</li>
</ol>

<ol><li>Cheng, L., Shahin, M. A., Mujah, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+key+environmental+conditions+on+microbially+induced+cementation+for+soil+stabilization%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Influence of key environmental conditions on microbially induced cementation for soil stabilization.</a> Journal of Geotechnical and Geoenvironmental Engineering. 143 (1), 04016083-04016091 (2016).</li>
<li>Whiffin, V. S., van Paassen, L. A., Harkes, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbial+carbonate+precipitation+as+a+soil+improvement+technique%5BTitle%5D)+AND+%22Geomicrobiology+Journal%22%5BJournal%5D)">Microbial carbonate precipitation as a soil improvement technique.</a> Geomicrobiology Journal. 24 (5), 417-423 (2007).</li>
<li>van Paassen, L. A., Ghose, R., van der Linden, T. J., van der Star, W. R., van Loosdrecht, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantifying+biomediated+ground+improvement+by+ureolysis%3A+large-scale+biogrout+experiment%5BTitle%5D)+AND+%22Journal+of+Geotechnical+And+Geoenvironmental+Engineering%22%5BJournal%5D)">Quantifying biomediated ground improvement by ureolysis: large-scale biogrout experiment.</a> Journal of Geotechnical And Geoenvironmental Engineering. 136 (12), 1721-1728 (2010).</li>
<li>Montoya, B. M., DeJong, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Stress-strain+behavior+of+sands+cemented+by+microbially+induced+calcite+precipitation%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Stress-strain behavior of sands cemented by microbially induced calcite precipitation.</a> Journal of Geotechnical and Geoenvironmental Engineering. 141 (6), 04015019(2015).</li>
<li>DeJong, J. T., Fritzges, M. B., Nüsslein, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbially+induced+cementation+to+control+sand+response+to+undrained+shear%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Microbially induced cementation to control sand response to undrained shear.</a> Journal of Geotechnical and Geoenvironmental Engineering. 132 (11), 1381-1392 (2006).</li>
<li>Zhao, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+improvement+of+engineering+properties+of+MICP-treated+soil+catalyzed+by+bacteria+and+urease%5BTitle%5D)+AND+%22Journal+of+Materials+in+Civil+Engineering%22%5BJournal%5D)">Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease.</a> Journal of Materials in Civil Engineering. 26 (12), 04014094(2014).</li>
<li>Castanier, S., Le Métayer-Levrel, G., Perthuisot, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ca-carbonates+precipitation+and+limestone+genesis%E2%80%94the+microbiogeologist+point+of+view%5BTitle%5D)+AND+%22Sedimentary+Geology%22%5BJournal%5D)">Ca-carbonates precipitation and limestone genesis—the microbiogeologist point of view.</a> Sedimentary Geology. 126 (1-4), 9-23 (1999).</li>
<li>Burne, R. A., Chen, Y. Y. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bacterial+ureases+in+infectious+diseases%5BTitle%5D)+AND+%22Microbes+and+Infection%22%5BJournal%5D)">Bacterial ureases in infectious diseases.</a> Microbes and Infection. 2 (5), 533-542 (2000).</li>
<li>Bernardi, D., DeJong, J. T., Montoya, B. M., Martinez, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bio-bricks%3A+biologically+cemented+sandstone+bricks%5BTitle%5D)+AND+%22Construction+and+Building+Materials%22%5BJournal%5D)">Bio-bricks: biologically cemented sandstone bricks.</a> Construction and Building Materials. 55, 462-469 (2014).</li>
<li>Li, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+fiber+addition+on+mechanical+properties+of+MICP-treated+sand%5BTitle%5D)+AND+%22Journal+of+Materials+in+Civil+Engineering%22%5BJournal%5D)">Influence of fiber addition on mechanical properties of MICP-treated sand.</a> Journal of Materials in Civil Engineering. 28 (4), 04015166(2015).</li>
<li>Achal, V., Kawasaki, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biogrout%3A+a+novel+binding+material+for+soil+improvement+and+concrete+repair%5BTitle%5D)+AND+%22Frontiers+in+Microbiology%22%5BJournal%5D)">Biogrout: a novel binding material for soil improvement and concrete repair.</a> Frontiers in Microbiology. 7, 314(2016).</li>
<li>Al Qabany, A., Soga, K., Santamarina, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+efficiency+of+microbially+induced+calcite+precipitation%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Factors affecting efficiency of microbially induced calcite precipitation.</a> Journal of Geotechnical and Geoenvironmental Engineering. 138 (8), 992-1001 (2011).</li>
<li>Lin, H., Suleiman, M. T., Brown, D. G., Kavazanjian, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanical+behavior+of+sands+treated+by+microbially+induced+carbonate+precipitation%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Mechanical behavior of sands treated by microbially induced carbonate precipitation.</a> Journal of Geotechnical and Geoenvironmental Engineering. 142 (2), 04015066(2015).</li>
<li>Lauchnor, E. G., Topp, D. M., Parker, A. E., Gerlach, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Whole+cell+kinetics+of+ureolysis+by+sporosarcina+pasteurii%5BTitle%5D)+AND+%22Journal+of+Applied+Microbiology%22%5BJournal%5D)">Whole cell kinetics of ureolysis by sporosarcina pasteurii.</a> Journal of Applied Microbiology. 118 (6), 1321-1332 (2015).</li>
<li>Nafisi, A., Montoya, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+framework+for+identifying+cementation+level+of+MICP-treated+sands%5BTitle%5D)+AND+%22IFCEE%22%5BJournal%5D)">A new framework for identifying cementation level of MICP-treated sands.</a> IFCEE. , conference paper (2018).</li>
<li>Zhao, Q., Li, L., Li, C., Zhang, H., Amini, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+full+contact+flexible+mold+for+preparing+samples+based+on+microbial-induced+calcite+precipitation+technology%5BTitle%5D)+AND+%22Geotechnical+Testing+Journal%22%5BJournal%5D)">A full contact flexible mold for preparing samples based on microbial-induced calcite precipitation technology.</a> Geotechnical Testing Journal. 37 (5), 917-921 (2014).</li>
<li>Bu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+Rigid+Full-Contact+Mold+for+Preparing+Biobeams+through+Microbial-Induced+Calcite+Precipitation%5BTitle%5D)+AND+%22Geotechnical+Testing+Journal%22%5BJournal%5D)">Development of a Rigid Full-Contact Mold for Preparing Biobeams through Microbial-Induced Calcite Precipitation.</a> Geotechnical Testing Journal. 42 (3), 656-669 (2018).</li>
<li>Li, M., Wen, K., Li, Y., Zhu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+oxygen+availability+on+microbially+induced+calcite+precipitation+%28MICP%29+treatment%5BTitle%5D)+AND+%22Geomicrobiology+Journal%22%5BJournal%5D)">Impact of oxygen availability on microbially induced calcite precipitation (MICP) treatment.</a> Geomicrobiology Journal. 35 (1), 15-22 (2018).</li>
<li>Martinez, B. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Experimental+optimization+of+microbial-induced+carbonate+precipitation+for+soil+improvement%5BTitle%5D)+AND+%22Journal+of+Geotechnical+and+Geoenvironmental+Engineering%22%5BJournal%5D)">Experimental optimization of microbial-induced carbonate precipitation for soil improvement.</a> Journal of Geotechnical and Geoenvironmental Engineering. 139 (4), 587-598 (2013).</li>
</ol>

We have nothing to disclose.

The MICP technique by immersion was presented in this paper. Soil samples were immersed into the batch reactor to get fully penetrated by cementation media in the MICP process. In this method, a full contact flexible mold, a rigid full contact mold, and a cored brick mold were applied to prepare MICP-treated samples.
Different molds can be designed for different geometry requirements. The fibrous structure of geotextile increased the contact area between sand and cementation media, which effec...

As a biological ground improvement technology, microbially induced calcite precipitation (MICP) is capable of improving engineering properties of soil. It has been used to enhance the strength, stiffness, and permeability of soil. The MICP technique has gained much attention for soil improvement worldwide1,2,3,4. Carbonate precipitation naturally happens and can be induced by nonpathogenic organisms that are native to the soil environment5. The MICP biogeochemical reaction is driven by the existence of ureolytic bacteria, urea and a calcium-rich solution5,6. Sporosarcina pasteurii is a highly active urease enzyme that catalyzes the reaction network towards precipitation of calcite7,8. The urea hydrolysis process produces dissolved ammonium (NH4+) and inorganic carbonate (CO32-). The carbonate ions react with calcium ions to precipitate as calcium carbonate crystals. The urea hydrolysis reactions are shown here:
<img alt="Urea hydrolysis equation, CO(NH₂)₂ + 2H₂O → 2NH₄⁺ + CO₃²⁻, chemical reaction formula." src="/files/ftp_upload/60059/60059equ01.jpg" data-altupdatedat="1788282455000" data-alt="Equation 1">
<img alt="Chemical reaction equation: Ca²⁺ + CO₃²⁻ → CaCO₃(s)↓; precipitation diagram." src="/files/ftp_upload/60059/60059equ02.jpg" data-altupdatedat="1788282455000" data-alt="Equation 2">
The precipitated CaCO3 can bond the sand particles together to improve the engineering properties of MICP-treated soil. The MICP technique has been applied in various applications, such as improvement of strength and stiffness of soil, repair of concrete, and environmental remediation9,10,11,12,13,14,15.
Zhao et al.16 developed an immersion method to prepare MICP-treated samples. A full contact flexible mold made of geotextile was used in this method. The precipitated CaCO3 distributed uniformly throughout their MICP-treated samples. Bu et al.17 developed a rigid full contact mold to prepare MICP-treated beam samples by an immersion method. The MICP-treated sample prepared by this method using a rigid full contact mold can form the suitable beam shape. The MICP-treated sample was divided into four and the CaCO3 contents were measured. The CaCO3 content ranged from 8.4 ± 1.5% to 9.4 ± 1.2% by weight, which indicated that the CaCO3 distributed uniformly in the MICP-treated samples by the immersion method. These MICP-treated samples also achieved better mechanical properties. These MICP-treated bio-specimens reached a 950 kPa flexure strength, which was similar to that of 20- 25% cement-treated samples (600- 1300 kPa). Li et al.10 added randomly distributed discrete fiber into the sandy soil and treated the soil by the MICP immersion method. They found that the shear strength, ductility, and failure strain of MICP-treated soil were enhanced obviously by adding appropriate fiber.
The immersion method for MICP has been continually improved10,16,17. This method can be used to prepare MICP-treated soil samples and MICP-treated prefabricated building materials, such as bricks and beams. Different geometry dimensions of sample preparation mold were developed. Fibers were added in the MICP-treated samples to enhance their properties. This detailed protocol was intended to document the immersion methods for MICP treatment.

<table><tbody><tr><td>Ammonium Chloride, &gt;99%</td><td>Bio-world</td><td>40100196-3 (705033)</td><td></td></tr><tr><td>Ammonium Sulfate</td><td>Bio-world</td><td>30635330-3</td><td></td></tr><tr><td>Calcium Chloride Dihydrate, &gt;99%</td><td>Bio-world</td><td>40300016-3 (705111)</td><td></td></tr><tr><td>Nutrient Broth</td><td>Bio-world</td><td>30620056-3</td><td></td></tr><tr><td>Sodium Bicarbonate, &gt;99%</td><td>Bio-world</td><td>41900068-3 (705727)</td><td></td></tr><tr><td>&lt;em&gt;Sporosarcina pasteurii&lt;/em&gt;</td><td>American Type Culture Collection</td><td>ATCC 11859</td><td></td></tr><tr><td>Synthetic fiber</td><td>FIBERMESH</td><td>Fibermesh 150e3</td><td></td></tr><tr><td>Tris-Base, Biotechnology Grade, &gt;99.7%</td><td>Bio-world</td><td>42020309-2 (730205)</td><td></td></tr><tr><td>Urea, USP Grade, &gt;99%</td><td>Bio-world</td><td>42100008-2 (705986)</td><td></td></tr><tr><td>Yeast Extract</td><td>Bio-world</td><td>30620096-3 (760095)</td><td></td></tr></tbody></table>

sandy soil improvement through microbially induced calcite precipitation (micp) by immersion

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion

The goal of this article is to develop an immersion method to improve the microbially induced calcite precipitation (MICP) treated samples. A batch reactor was assembled to immerse soil samples into cementation media. The cementation media can freely diffuse into the soil samples in the batch reactor instead of cementation media being injected. A full contact flexible mold, a rigid full contact mold, and a cored brick mold were used to prepare different soil sample holders. Synthetic fibers and natural fibers were selected to reinforce the MICP-treated soil samples. The precipitated CaCO3 in different areas of the MICP-treated samples was measured. The CaCO3 distribution results demonstrated that the precipitated CaCO3 was distributed uniformly in the soil sample by the immersion method.

Figure 7 shows the distribution of precipitated CaCO3 throughout the MICP-treated sample. The MICP-treated sample was divided into three different areas. The CaCO3 content in each area was tested by the acid washing method. To dissolve precipitated carbonates, the dry MICP-treated samples were washed in a HCl solution (0.1 M), then rinsed, drained, and oven-dried for 48 hours. The difference value between the masses of samples before and after acid washing was considere...

Here, microbially induced calcite precipitation (MICP) technology is presented to improve soil properties by immersion.

The goal of this article is to develop an immersion method to improve the microbially induced calcite precipitation (MICP) treated samples. A batch ...

sandy-soil-improvement-through-microbially-induced-calcite

Research

JoVE Journal

Engineering

Source: Bhaduri, S., et al., Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii. J. Vis. Exp. (2016)This video demonstrates the method of enriching Sporosarcina pasteurii with urea and calcium chloride to trigger urease activity, leading to the formation of visible calcite crystals through Microbiologically Induced Calcite Precipitation, or MICP.

Microbiologically Induced Calcite Precipitation by Sporosarcina pasteurii

Protocols for microbiologically induced calcite precipitation (MICP) using the bacterium Sporosarcina pasteurii are presented here. The precipitated calcium carbonate was characterized through optical microscopy and scanning electron microscopy (SEM). It is also shown that exposure to MICP increases the compressive strength of sponge.

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

A protocol for the parallel production of precipitated calcium carbonate and zeolitic material from blast furnace slag via mineral carbonation and alkaline hydrothermal conversion, respectively, is presented. The performance of the zeolitic material towards nickel adsorption is tested.

Two-way Valorization of Blast Furnace Slag: Synthesis of Precipitated Calcium Carbonate and Zeolitic Heavy Metal Adsorbent

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Spatial patterns of soil erosion and deposition can be inferred from differences in ground elevation mapped at appropriate time increments. Such changes in elevation are related to changes in near-surface soil carbonates. Repeatable methods for field and laboratory measurements of these quantities and data analysis methods are described here.

Measuring and Mapping Patterns of Soil Erosion and Deposition Related to Soil Carbonate Concentrations Under Agricultural Management

We describe a protocol for the precipitation and characterization of calcium carbonate crystals that form in the presence of biopolymers.

Calcium Carbonate Formation in the Presence of Biopolymeric Additives

Accurate quantification of phosphorus (P) desorption potential in saturated soils and sediments is important for P modeling and transport mitigation efforts. To better account for in situ soil-water redox dynamics and P mobilization under prolonged saturation, a simple approach was developed based on repeated sampling of laboratory microcosms.

Measuring Phosphorus Release in Laboratory Microcosms for Water Quality Assessment

The verification method described here is adaptable for monitoring pedogenic inorganic carbon sequestration in various agricultural soils amended with alkaline earth metal silicate-containing rocks, such as wollastonite, basalt, and olivine. This type of validation is essential for carbon credit programs, which can benefit farmers that sequester carbon in their fields.

Monitoring Pedogenic Inorganic Carbon Accumulation Due to Weathering of Amended Silicate Minerals in Agricultural Soils.

The present protocol describes a microfluidic platform to study biofilm development in quasi-2D porous media by combining high-resolution microscopy imaging with simultaneous pressure difference measurements. The platform quantifies the influence of pore size and fluid flow rates in porous media on bioclogging.

A Microfluidic Platform to Study Bioclogging in Porous Media

Here we present the construction and operation of an experimental setup to enhance mineral weathering through the activity of soil organisms while concurrently manipulating abiotic variables known to stimulate weathering. Representative results from the functioning of the setup and sample analyses are discussed together with points for improvement.

Design and Construction of an Experimental Setup to Enhance Mineral Weathering through the Activity of Soil Organisms

Sporosarcina pasteurii is a ureolytic bacterium that breaks down urea into carbonate and ammonium. The carbonate combines with calcium to form calcium carbonate, creating a crystal lattice that anchors surrounding particles together to produce biocement. This is a convenient protocol for using 3D-printed molds to create biocement bricks suitable for compression testing.

Production and Analysis of Sporosarcina pasteurii Biocement Bricks Using Custom 3D-Printed Molds for Unconfined Compression Tests

Learn microbially induced calcite precipitation by immersion to improve sandy soil strength and stability. This method enables uniform calcium carbonate precipitation for enhanced mechanical testing of treated samples.

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion

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