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JoVE Journal
Immunology and Infection

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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
 

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Article doi: 10.3791/60060
September 20th, 2019 Usage Statistics

Summary September 20th, 2019

In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

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