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Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry
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Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry

Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry

DOI:
10.3791/60086-v

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05:15 min

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October 20, 2019

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1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-sen University

Chapters

  • 00:04Title
  • 01:18Dissection of Eyeball with Intact Ocular Surface and Eyelids
  • 03:52Results: Histological Examination and Immunohistochemistry of Obtained Integral Ocular Surface Tissue
  • 04:48Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for the isolation of the mouse eyeball with eyelid, ocular surface, anterior and posterior segments in relatively intact position.

Tags

Keywords: Ocular SurfaceHistological ExaminationImmunohistochemistryMouseDissectionEyeballOrbital BonesEyelidsLensRetinaOcular Surface Epithelial SheetHistologyImmunostainingEuthanasiaOptic NerveBrainParaformaldehydePBSSkullOrbital BonesEthmoidFrontalMaxillary BonesForceps

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