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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Article DOI: 10.3791/60104-v • 11:01 min • January 18th, 2020
January 18th, 2020 •
*1, *2, 2, 1, 1, 1, 2, 2, 1,2,3, 1
1Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, 2School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, 3School of Medical and Health Sciences, Edith Cowan University
* These authors contributed equally

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Summary
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Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

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Immunoglobulin G N-glycan Analysis Ultra-performance Liquid Chromatography Sensitivity Specific Information Ease Of Use Low Cost High Reproducibility Glycan Isomers Plasma Protein Measurements Medicine Impure State Testing Cross-bound Protein Pure State Protein Analysis Experimental Process Standardization Sample Preparation Centrifuge Protein G Monolithic Plate Control Sample Dilution Ratio Polypropylene Filter Plate
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