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Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

DOI:
10.3791/60112-v

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11:42 min

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November 04, 2019

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, , , ,
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, 2Wallenberg Center for Molecular,Lund University, 3Center for Neuroscience and Cell Biology,University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Title
  • 00:41Lentiviral Production
  • 05:28Hemogenic Reprogramming
  • 07:34Optimization of Fibroblast Expansion for ChIP-Seq Analysis at the Onset of Hemogenic Reprogramming
  • 08:59Results: Hemogenic Fate Induction and Chromatin ChIP-Seq Analysis
  • 10:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.

Tags

Keywords: Hemogenic ReprogrammingHuman FibroblastsTranscription FactorsLentiviral ProductionFibroblast TransductionCell CultureHEK293T CellsTransfer PlasmidsPackaging VectorEnvelope VectorCalcium ChlorideHematopoietic Stem And Progenitor CellsStem Cell Transplantation

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