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JoVE Journal
Developmental Biology

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Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
 

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Article doi: 10.3791/60112
November 4th, 2019

Summary November 4th, 2019

This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.

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