Name: Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors (Video) | JoVE Journal
Uploaded: 2019-11-04T13:00:00.000+00:00
Duration: 11 min 42 s
Description: Learn hemogenic reprogramming of human dermal fibroblasts using transcription factors GATA2, GFI1B, and FOS to generate hematopoietic stem and progenitor cells. This method enables patient-specific cell production for transplantation research.

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5 minutes

The Knut and Alice Wallenberg foundation, the Medical Faculty at Lund University and Region Sk&#229;ne are acknowledged for generous financial support. This work was supported by a grant from Olle Engkvists Stiftelse (194-0694 to Filipe Pereira) and PhD scholarships from Funda&#231;&#227;o para a Ci&#234;ncia e Tecnologia (PTDC/BIM-MED/0075/2014 to Filipe Pereira, and SFRH/BD/135725/2018 and SFRH/BD/51968/2012 to Rita Alves and Andreia Gomes).&#160;This study was also supported by funds from NIH and NYSTEM (1R01HL119404 and C32597GG to Kateri A. Moore).

This protocol was performed according to Lund University&#39;s human research ethics committee guidelines and should be done in accordance with individual institutional guidelines.
1. Reagent Preparation
<ol>
	<li>For Dulbecco&#39;s modified Eagle's medium (DMEM)/20% fetal bovine serum (FBS), mix high glucose DMEM containing sodium pyruvate with 20% FBS, 1% penicillin-streptomycin (pen/strep), 1% L-glutamine, 1% non-essential amino acids and 10-4-M 2-mercaptoethanol.</li>
	<li>For complete DMEM, mix high glucose DMEM containing sodium pyruvate with 10% FBS, 1% Pen/strep and 1% L-glutamine.</li>
	<li>For hematopoietic medium, mix hematopoietic medium (Table of Materials) with 10-6 M hydrocortisone and 1% pen/strep.</li>
	<li>Use phosphate-buffered saline (PBS) without calcium or magnesium.</li>
</ol>
2. Human Dermal Fibroblast Isolation
NOTE: HDFs can be purchased from certified suppliers (Table of Materials). In that case, expand fibroblasts and use them directly in reprogramming experiments (section 4). Alternatively, HDFs can be isolated from donors. If fibroblasts are isolated from different donors, keep the samples separated from each other at all steps of the protocol. Label plates/wells and collection tubes with the identification number of each donor.
<ol>
	<li>Obtain HDFs from 3 mm round skin punch biopsies performed by qualified physicians.</li>
	<li>Coat three wells of a tissue culture-treated 6-well plate with 500 &#956;L of 0.1% gelatin and incubate for 20 min at 37 &#176;C.</li>
	<li>Aspirate the remaining gelatin solution and add 750 &#956;L of DMEM/20% FBS to each well. The entire surface of the well should be covered with medium.</li>
	<li>Add 1.5 mL of DMEM/20% FBS to the inside surface of a sterile 100 mm petri dish lid and spread the drop with the aid of a 5 mL serological pipette.</li>
	<li>Place the skin biopsy in the medium on the lid with sterilized forceps.</li>
	<li>Dissect the skin biopsy into nine identical sections, using one sterilized scalpel to hold the biopsy in place and a second scalpel to cut.</li>
	<li>Place three biopsy pieces per well using pointed forceps. Make sure the pieces attach to the bottom of the well.</li>
	<li>Lay a 22 mm coverslip on top of the pieces and apply some pressure.</li>
	<li>Incubate the plate at 37 &#176;C, 5% CO2, for a week. Check cells daily and add 200 &#956;L of DMEM/20% FBS every 2 days to replace evaporated medium.</li>
	<li>After one week, add up to 2 mL of DMEM/20% FBS and replace medium every 2&#8722;3 days.</li>
	<li>Passage cells at 1:4 ratio when wells are confluent (about 4&#8722;8 weeks).
	<ol>
		<li>Prepare 0.1% gelatin coated tissue culture-treated 6-well plates.</li>
		<li>Aspirate medium from wells at 80% confluency and wash once with 1 mL of PBS.</li>
		<li>Remove coverslip with sterile forceps and place the coverslip into a new well of a 6-well plate, with the tissue side up. 
		NOTE: Cells that remained attached to the coverslip will also be harvested.</li>
		<li>Add 500 &#956;L of dissociation solution (Table of Materials) per well (including wells with the coverslips) and incubate at 37 &#176;C, 5% CO2 for 5&#8722;10 min. Check when cells start to rise from the bottom of the well and inactivate the dissociation solution by adding 500 &#956;L of DMEM/20% FBS to each well.</li>
		<li>Collect fibroblasts from all the wells into a 15 mL conical tube. Add extra medium to the wells to collect the remaining cells. Centrifuge the tube at 350 x g for 5 min.</li>
		<li>In the meantime, add 500 &#956;L of DMEM/20% FBS to each well of previously gelatin coated plates.</li>
		<li>Aspirate medium and resuspend fibroblasts in 6 mL of DMEM/20% FBS.</li>
		<li>Add 500 &#956;L of fibroblast suspension to each well (two 6-well plates per sample/donor in total). Incubate cells overnight at 37 &#176;C, 5% CO2.</li>
	</ol>
	</li>
	<li>On the next day, add 1 mL of DMEM/20% FBS to each well. Replace medium with 2 mL of DMEM/20% FBS every 2&#8722;3 days until wells are 80% confluent.</li>
	<li>Repeat section 2.11 for three confluent wells until third passage is reached.</li>
	<li>Freeze fibroblasts from confluent wells (passages 1 and 3).
	<ol>
		<li>Aspirate medium from the wells and wash once with 1 mL of PBS.</li>
		<li>Dissociate and collect fibroblasts as described in steps 2.11.4 and 2.11.5.</li>
		<li>Count cells with a hemocytometer and centrifuge the tube at 350 x g for 5 min.</li>
		<li>After centrifugation, aspirate medium and resuspend fibroblasts in FBS with 10% DMSO at a density of 5 x 105 cells/mL.</li>
		<li>Add 1 mL of the cell suspension per cryovial and freeze cells overnight at -80 &#176;C using a freezing container. Move vials to -150 &#176;C (liquid nitrogen) for long-term storage.</li>
	</ol>
	</li>
</ol>
3. Lentiviral Production
<ol>
	<li>Grow HEK293T cells in a 100 mm tissue culture-treated dish with 10 mL of complete DMEM, at 37 &#176;C, 5% CO2, until confluency is reached.</li>
	<li>On the day prior to transfection, aspirate medium and wash the dish carefully with 5 mL of PBS.</li>
	<li>After removing PBS, add 1.5 mL of dissociation solution and incubate at 37 &#176;C, 5% CO2 for 5&#8722;10 min to dissociate cells from the dish. 
	NOTE: It is recommended to warm both PBS and dissociation solution before using, so that cells do not suffer a thermal shock.</li>
	<li>Inactivate dissociation solution with 3 mL of complete DMEM and transfer the cell suspension to a 15 mL conical tube. Wash the dish with 5 mL of complete DMEM to remove remaining attached cells and transfer this volume to the 15 mL conical tube.</li>
	<li>Centrifuge cell suspension at 350 x g for 5 min.</li>
	<li>Aspirate supernatant and split the cell pellet evenly between six 100 mm tissue culture-treated dishes in a final volume of 10 mL of complete DMEM per dish. Cells should be approximately 60% confluent by the time of transfection.</li>
	<li>On the next day, transfect cells with the plasmid mixes as follows: 
	NOTE: This part of the protocol describes the production of lentiviruses in one 100 mm tissue culture-treated dish per plasmid mix. To obtain higher volumes of lentiviral supernatant for concentration, use at least four 100 mm HEK293T cell culture dishes per mix.
	<ol>
		<li>In a 15 mL conical tube, add 10 &#956;g of the three transfer plasmids together: 3.33 &#956;g of pFUW-tetO-GATA2 (Addgene plasmid #125028)14, 3.33 &#956;g of pFUW-tetO-GFI1B (Addgene #125597)14 and 3.33 &#956;g of pFUW-tetO-FOS (Addgene #125598)14, plus 10 &#956;g of the 2nd generation psPAX2 packaging vector encoding the Gag, Pol, Tat and Rev genes (Addgene #12260) and 5 &#956;g of pMD2.G envelope vector encoding the VSV-G gene (Addgene #12259). Add water up to 500 &#956;L.</li>
		<li>In two new 15 mL conical tubes add 10 &#956;g of FUW-M2rtTA plasmid (Addgene #20342)17, 10 &#956;g of psPAX2 packaging vector and 5 &#956;g of pMD2.G envelope vector to each tube. Add water up to 500 &#956;L. One tube is going to be used as a control.</li>
		<li>To each tube add 62.5 &#956;L of 2 M CaCl2. Next, release bubbles into each mixture using a Pasteur pipet inserted into a pipet controller. While bubbles are forming, pipette 500 &#956;L of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) buffered saline (pH 7.1, 25 &#176;C), with a P1000 pipette, drop-wise against the Pasteur pipet and onto the mixture.</li>
		<li>Incubate tubes at room temperature for at least 15 min. The mixtures will appear slightly cloudy after some time.</li>
	</ol>
	</li>
	<li>Meanwhile, aspirate medium from HEK293T cell dishes (passaged the day before) and add 10 mL of complete DMEM without antibiotics. Be careful and add medium slowly as HEK293T cells are semi-adherent.</li>
	<li>Distribute each individual mixture (approximately 1 mL) evenly and drop-wise into separate dishes and incubate overnight at 37 &#176;C, 5% CO2.</li>
	<li>Replace medium with 4 mL of complete DMEM, 24 h after incubation. Incubate overnight at 37 &#176;C, 5% CO2. If available, incubate instead at 32 &#176;C, 5% CO2, as the reduced temperature will increase the half-life of the lentiviral particles.</li>
	<li>Collect supernatant with lentiviral particles three times to a 50 mL conical tube. Do not mix different lentiviral particles at this point. Each dish will result in 12 mL of lentiviral supernatant. Four dishes of the same viral preparation fit into one 50 mL conical tube. 
	CAUTION: Perform lentiviral collection in a biosafety level-2 laboratory in a laminar flow hood dedicated for lentiviral work and place viral contaminated waste (tubes, tips, dishes) in an appropriate container for biohazardous materials.
	<ol>
		<li>Do the first collection 16 h after the last incubation and add 4 mL of complete DMEM. Incubate at 37 &#176;C, 5% CO2.</li>
		<li>Do the second collection 8 h after the first to the same tube, add 4 mL of complete DMEM and incubate at 37 &#176;C, 5% CO2.</li>
		<li>Do the third collection 16 h after the second to the same tube and discard the dishes. 
		NOTE: Store lentiviral supernatants at 4 &#176;C after each collection.</li>
	</ol>
	</li>
	<li>Filter each lentiviral supernatant using a 0.45 &#956;m low-protein binding filter with a cellulose acetate membrane (Table of Materials) to a clean tube.</li>
	<li>Add a maximum of 15 mL of filtered supernatant to a centrifugal filter unit with a regenerated cellulose membrane (Table of Materials) and spin at 4,000 x g for 25 min, at 4 &#176;C. Discard flow-through. A viscous liquid containing lentiviruses will remain in the filter unit.</li>
	<li>Repeat step 3.13 by adding 15 mL of supernatant on top of the filter unit, until there is no more lentiviral supernatant left. 
	NOTE: When there are only a few milliliters of supernatant to concentrate, decrease the spinning time to 10 min. If there is still extra liquid (non-viscous) on the filter, centrifuge for an additional 10 min.</li>
	<li>Make aliquots (50&#8722;200 &#956;L depending on the initial supernatant volume) of each type of concentrated lentiviruses and store at -80 &#176;C for long-term storage (1&#8722;2 years) or at 4 &#176;C for short-term storage (1&#8722;2 weeks). 
	NOTE: Concentrated or non-concentrated lentiviruses can also be used fresh. Do not re-freeze and thaw as this results in reduced titer.</li>
</ol>
4. Hemogenic Reprogramming
NOTE: Use HDFs with a passage number of three (P3) or higher (until P10) to perform reprogramming experiments.
<ol>
	<li>Coat a 100 mm tissue culture-treated dish with 5 mL of 0.1% gelatin and incubate at 37 &#176;C for 20 min. Aspirate the remaining gelatin solution.</li>
	<li>Thaw a fibroblast vial and plate cells in the 0.1% gelatin-coated dish. Incubate overnight at 37 &#176;C, 5% CO2. If necessary, expand fibroblasts for a longer period of time until the desired passage and confluency are reached.</li>
	<li>Coat a 6-well tissue culture-treated plate with 500 &#956;L of 0.1% gelatin solution and incubate at 37 &#176;C for 20 min. Remove extra gelatin.</li>
	<li>Plate HDFs at a density of 150,000 cells per plate (25,000 cells per well) in 2 mL of complete DMEM per well. Incubate overnight at 37 &#176;C, 5% CO2, to allow cell attachment.</li>
	<li>Replace medium with 2 mL of complete DMEM plus 8 &#956;g/mL polybrene. Prepare a 1:1 ratio mix of pool-produced TF lentiviruses and M2rtTA in a new microcentrifuge tube. 
	NOTE: In this protocol, pool-production of lentiviruses for the three TFs is performed, which, in authors&#39; hands, results in higher reprogramming efficiency. Alternatively, it is suggested to perform a titration of the individual lentiviral particles by qPCR18, on a standard cell line. This will be used to define the volume of individual viruses necessary to meet a multiplicity of infection (MOI) optimal for co-transduction and hemogenic reprogramming.</li>
	<li>Distribute 10 to 100 &#956;L of lentiviral mixture per well, to transduce HDFs. This is day -2 of reprogramming. 
	NOTE: Defining the optimal volume of lentiviral mix for efficient reprogramming, without compromising cell viability, requires optimization (see Supplementary Figure 1 for more details). HDFs with more than 7 passages may require higher volumes of viruses than cells with lower passages.</li>
	<li>After 16 h of incubation, remove viruses and add complete DMEM. Allow cells to recover for 6&#8722;8 h.</li>
	<li>After recovery, aspirate medium and add 2 mL of complete DMEM with 8 &#956;g/mL polybrene.</li>
	<li>Do a second transduction as described in step 4.6 and incubate at 37 &#176;C, 5% CO2 for 16 h. This is day -1 of reprogramming. The lentiviral mix can be prepared on day -2 for both transductions and kept at 4 &#176;C.</li>
	<li>On the next day, remove the viruses and add complete DMEM supplemented with 1 &#956;g/mL DOX. This is day 0 of reprogramming. Incubate at 37 &#176;C, 5% CO2 for 48 h.</li>
	<li>At day 2 of reprogramming, split each well at 1:2 ratio.
	<ol>
		<li>Aspirate medium and wash cells with 1 mL of PBS.</li>
		<li>Aspirate PBS and dissociate cells with 500 &#956;L of dissociation solution. Incubate 5&#8722;10 min at 37 &#176;C, 5% CO2.</li>
		<li>Inactivate the dissociation solution with 1 mL of complete DMEM and collect cells into a conical tube. Centrifuge at 350 x g for 5 min.</li>
		<li>Resuspend the pellet in hematopoietic medium (see step 1.3), supplemented with 1 &#956;g/mL DOX, and plate cells into new tissue culture-treated 6-well plates coated with 0.1% gelatin to a final volume of 2 mL per well.</li>
	</ol>
	</li>
	<li>Change medium (hematopoietic medium plus DOX) twice a week for the duration of the reprogramming cultures (25 days).</li>
	<li>Analyze resulting reprogrammed cells at different time points by brightfield or fluorescence microscopy (see Supplementary Figure 2), flow cytometry, bulk and single-cell RNA sequencing, and transplantation assays for the acquisition of hematopoietic morphology, presence of endothelial and hematopoietic markers, acquisition of endothelial/hematopoietic gene expression profile and regeneration capacity14.</li>
</ol>
5. Optimization of Fibroblast Expansion for ChIP-seq Analysis at&#160;the Onset of Hemogenic Reprogramming
<ol>
	<li>Plate 300,000 HDFs (&#60;P8) in 0.1% gelatin coated tissue culture-treated 6-well plates with complete DMEM to a final volume of 2 mL per well. Incubate overnight at 37 &#176;C, 5% CO2.</li>
	<li>On the following day, replace medium with complete DMEM supplemented with 8 &#956;g/mL polybrene.</li>
	<li>Transduce cells with individual factors: pFUW-tetO-FOS14, pLV-tetO-HA-GFI1B (Addgene #125599)14 and pFUW-tetO-3xFLAG-GATA2 (Addgene #125600)14 or with a pool of the three factors, plus FUW-M2rtTA at 1:1 ratio. Use 10&#8722;20 &#956;L total virus (individual TF + M2rtTA or three TFs + M2rtTA). Incubate cells overnight at 37 &#176;C, 5% CO2. 
	NOTE: It is recommended to use twelve 6-well plates per condition (for each individual TF and the three TFs combined).</li>
	<li>Remove lentiviruses and add complete DMEM 16 h after the first transduction. Let cells recover for 6&#8722;8 h.</li>
	<li>Transduce cells a second time with the same amount of virus per condition and incubate at 37 &#176;C, 5% CO2.</li>
	<li>On the next day remove viruses and add complete DMEM. Incubate at 37 &#176;C, 5% CO2 for 24 h.</li>
	<li>Re-plate each well into a 0.1% gelatin coated tissue culture-treated 100 mm dish with complete DMEM to a final volume of 10 mL per dish. This represents approximately a 1:6 passage.</li>
	<li>Allow cells to grow for 6 days at 37 &#176;C, 5% CO2.</li>
	<li>On day 6 after re-plating, aspirate medium and add complete DMEM with 1 &#956;g/mL DOX. Incubate cells at 37 &#176;C, 5% CO2 for 2 days.</li>
	<li>Collect fibroblasts and analyze genomic binding sites of the three TFs transduced individually or in combination, by ChIP-seq 2 days after DOX supplementation14. 
	NOTE: The final seventy-two 100 mm dishes will contain between 20&#8722;50 x 106 cells, sufficient to perform ChIP-seq experiments and replicates.</li>
</ol>

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<li>Pereira, C., Lemischka, I. R., Moore, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((From+blood+to+blood%E2%80%99%3A+de-differentiation+of+hematopoietic+progenitors+to+stem+cells%5BTitle%5D)+AND+%22EMBO+Journal%22%5BJournal%5D)">From blood to blood’: de-differentiation of hematopoietic progenitors to stem cells.</a> EMBO Journal. 33, 1511-1513 (2014).</li>
<li>Nolan, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Molecular+Signatures+of+Tissue-Specific+Microvascular+Endothelial+Cell+Heterogeneity+in+Organ+Maintenance+and+Regeneration%5BTitle%5D)+AND+%22Developmental+Cell%22%5BJournal%5D)">Molecular Signatures of Tissue-Specific Microvascular Endothelial Cell Heterogeneity in Organ Maintenance and Regeneration.</a> Developmental Cell. 26, 204-219 (2013).</li>
<li>Batta, K., Florkowska, M., Kouskoff, V., Lacaud, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Direct+Reprogramming+of+Murine+Fibroblasts+to+Hematopoietic+Progenitor+Cells%5BTitle%5D)+AND+%22Cell+Reports%22%5BJournal%5D)">Direct Reprogramming of Murine Fibroblasts to Hematopoietic Progenitor Cells.</a> Cell Reports. 9, 1871(2014).</li>
<li>Cheng, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reprogramming+mouse+fibroblasts+into+engraftable+myeloerythroid+and+lymphoid+progenitors%5BTitle%5D)+AND+%22Nature+Communications%22%5BJournal%5D)">Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors.</a> Nature Communications. 7, 1-15 (2016).</li>
</ol>

The authors have nothing to disclose.

In this article, a method is described to generate hematopoietic progenitor cells directly from human fibroblasts, which go through an HP cell intermediate, similarly to definitive HSCs14.
Pool-production of lentiviral particles encoding GATA2, GFI1B and FOS was preferred over individual production, since in our hands it results in higher reprogramming efficiencies (unpublished data). Lentiviruses, as members of the Retroviridae&#160;family, normally contain two copies of positive single-stranded RNA19. The increased reprogramming efficiency may be due to packaging of two different transgenes in the same lentiviral particle, resulting in increased number of cells co-transduced with the three transcription factors. To ensure the success of this protocol, it is necessary to transduce HDFs with adequate amount of virus depending on the cell passage to obtain an optimal balance between reprogramming efficiency and cell viability, as recommended in step 4.6. Moreover, fresh non-concentrated viruses can be used. It is recommended to transduce cells with 0.5-3 mL of 3TFs pool and M2rtTA. Also, cell density should be adjusted according to the application. 150&#160;000 HDFs per 6-well plate (step 4.4) provided the optimal density to perform FACS, transplantation and flow cytometry analysis of reprogrammed cells. For ChIP-seq experiments, more cells were required from the beginning (step 5.1). It is important to check cells regularly for morphological changes and replace hematopoietic medium twice a week to support the emergence of induced hematopoietic cells. Addition of hematopoietic cytokines or co-culture in feeder layers may increase reprogramming efficiency.
With this method, we can identify new hematopoietic markers that are dynamically expressed during hemogenic reprogramming. CD9, which was shown to be up-regulated in reprogrammed cells at the transcriptional level14, is rapidly expressed at the cell surface in the initial phases of reprogramming together with CD49f and CD143, serving as a novel marker of human HSC precursors. We also show that ITGA6 and ACE are direct targets of GATA2 during the initial stages of hemogenic reprogramming, in addition to CD9 and CD3414, providing a direct mechanistic link between human hemogenic precursor phenotype and GATA2.
One advantage of this system resides in the use of relatively homogeneous fibroblast cultures. While PSCs are easily expanded and maintained in vitro, differentiation protocols generate heterogeneous populations that include hematopoietic progenitors, which engraft poorly5,6,7. Moreover, there is a risk of tumorigenesis when transplanting PSC-derived HSPCs, since undifferentiated PSCs may still remain in culture even after employing differentiation protocols. Alternatively to fibroblasts, direct reprogramming to HSCs has been applied to blood-committed progenitors20 and endothelial cells21. However, starting with blood-restricted progenitor cells hinders therapeutic application of the resulting HSCs if the patient carries mutations that affect the stem/progenitor hematopoietic population22. In the case of endothelial cells, these are more difficult to obtain compared to fibroblasts, and constitute a very heterogeneous cell population in terms of phenotype, function and structure, which are organ-dependent23. Other studies have succeeded in reprogramming mouse fibroblasts into engraftable hematopoietic progenitors24,25 yet, so far, no other protocol describes the generation of HSPC-like cells from human fibroblasts.
This approach, coupled with pharmacological inhibition, gene knock-out, or knock-down permits to define individual or combination of factors that are required to directly induce human HSCs. Employing high efficiency screening methodologies based on recent CRISPR-Cas9 technologies in HDFs prior to reprogramming, represents an exciting endeavor for defining novel regulators of human definitive hematopoiesis. In the future, reprogramming non-blood related human cell types such as fibroblasts will serve as a platform to generate healthy patient-tailored hematopoietic progenitor cells for clinical applications.

An erratum was issued for: <a href="https://www.jove.com/video/60112/hemogenic-reprogramming-human-fibroblasts-enforced-expression">Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors</a>. The author affiliations were updated.
The second author affiliation was&#160;updated from:
2Wallenberg Center for Molecular, Lund University
to:
2Wallenberg Center for Molecular Medicine, Lund University

An erratum was issued for: <a href="https://www.jove.com/video/60112/hemogenic-reprogramming-human-fibroblasts-enforced-expression">Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors</a>. The author affiliations were updated.

Erratum: Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Definitive hematopoietic stem and progenitor cells (HSPCs) emerge in the aorta-gonad-mesonephros (AGM) region and placenta from endothelial precursors with hemogenic capacity, through an endothelial-to-hematopoietic transition (EHT)1,2. Hemogenic precursors (HPs) express both endothelial and hematopoietic markers, but their precise identification remains elusive, particularly in the human system. Despite being a relatively conserved process in mammals, hematopoietic stem cell (HSC) development still differs significantly between humans and mouse models3,4. Therefore, in vitro approaches to recapitulate human HSC development are needed.
Differentiation of pluripotent stem cells (PSCs) to HSCs, although promising, has met limited success over the past 20 years, mostly due to the available differentiation protocols, which result in primitive hematopoietic progenitors with poor engraftment ability5,6,7. Alternatively, direct cell reprogramming methodologies have been applied to generate HSPC-like cells from multiple cell types, using transcription factors (TFs)8,9. In particular, the overexpression of three TFs, Gata2, Gfi1b and cFos, converted mouse embryonic fibroblasts into HSPCs through an HP intermediate with a defined phenotype (Prom1+Sca-1+CD34+CD45-)10. This process resembled the EHT that occurs in the embryo and placenta, during specification of definitive hematopoiesis. This phenotype enabled the identification and isolation of a population of HPs in the mouse placenta that after short-term culture and Notch activation generated serially transplantable HSCs11.
So far, no phenotype has been established that distinguishes human HSCs from their precursors, but some molecules are known to be expressed in emerging HSCs. Integrin alpha 6 (ITGA6 or CD49f) is highly expressed in long-term repopulating HSCs, the most immature cells in the HSC compartment12, and angiotensin-converting enzyme (ACE or CD143) is present in CD34 negative hematopoietic precursors in embryonic blood-forming tissues13.
Recently, we have demonstrated that human versions of the three TFs, GATA2, FOS and GFI1B reprogram human dermal fibroblasts (HDFs) into HPs with short-term engraftment capacity14. In the initial phases of reprogramming, GATA2 engages open chromatin and recruits GFI1B and FOS to repress fibroblast genes and activate endothelial and hematopoietic genes. Induced cells highly expressed CD49f and ACE, and contained a small percentage of cells expressing the HSPC marker CD34. The CD9 gene, which is expressed in HSCs15 and is important for HSC homing16, was shown to be a direct target of GATA2 and among the most up-regulated genes in reprogrammed cells14. CD9 may therefore constitute an additional marker for HPs of human definitive hematopoiesis.
In this protocol, we describe the generation of HSPC-like cells from human fibroblasts through enforced expression of GATA2, GFI1B and FOS, as well as an adapted method for chromatin immunoprecipitation (ChIP)-sequencing (seq) analysis at the onset of reprogramming. TFs were encoded in a doxycycline (DOX)-inducible lentiviral vector (pFUW-tetO) that contains a tetracycline response element (TRE) and a minimal CMV promoter, and were transduced together with a constitutive vector containing the reverse tetracycline transactivator protein (pFUW-M2rtTA). When DOX (analog of tetracycline) is added after transduction, it binds to the rtTA protein which interacts with the TRE allowing TF transcription (Tet-On system). The procedure requires 25 days to complete. For ChIP-seq experiments, HDFs were transduced with tagged versions of GATA2 (pFUW-tetO-3xFLAG-GATA2) and GFI1B (pLV-tetO-HA-GFI1B), plus pFUW-tetO-FOS and TF binding sites were analyzed two days after DOX supplementation.
Ultimately, hemogenic reprogramming of human fibroblasts provides an in vitro tractable system to study the mechanisms underlying human developmental hematopoiesis and a potential source of patient-specific HSPCs for future clinical application.

<table><tbody><tr><td>0.45 &amp;mu;m low-protein binding filter, 150 mL Bottle Top Vacuum Filter</td><td>Corning</td><td>#430625</td><td></td></tr><tr><td>2-Mercaptoethanol</td><td>Sigma-Aldrich</td><td>#M6250</td><td></td></tr><tr><td>Alexa Fluor 488 anti-human CD34 clone 581</td><td>BioLegend</td><td>#343518</td><td></td></tr><tr><td>BD Pharmingen APC Mouse Anti-Human Angiotensin Converting Enzyme (CD143) clone BB9</td><td>BD Biosciences</td><td>#557929</td><td></td></tr><tr><td>BES buffered saline</td><td>Sigma-Aldrich</td><td>#14280</td><td></td></tr><tr><td>Calcium chloride (CaCl&lt;sub&gt;2&lt;/sub&gt;)</td><td>Sigma-Aldrich</td><td>#449709</td><td></td></tr><tr><td>Centrifugal filter unit, Amicon Ultra-15 Centrifugal Filter Unit</td><td>Sigma-Aldrich</td><td>#UFC903096</td><td></td></tr><tr><td>Dissociation solution, TrypLE Express Enzyme (1x) no phenol red</td><td>Gibco</td><td>#12604-021</td><td></td></tr><tr><td>Doxycycline hyclate (DOX)</td><td>Sigma-Aldrich</td><td>#D9891</td><td></td></tr><tr><td>eBioscience CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), PE-Cyanine7</td><td>Invitrogen</td><td>#25-0495-82</td><td></td></tr><tr><td>FUW-M2rtTA</td><td>Addgene</td><td>#20342</td><td></td></tr><tr><td>Gelatin from Porcine Skin Type A</td><td>Sigma-Aldrich</td><td>#G1890</td><td></td></tr><tr><td>Gibco L-Glutamine (200 mM)</td><td>ThermoFisher Scientific</td><td>#25030-024</td><td></td></tr><tr><td>Gibco MEM Non-Essential Amino Acids Solution (100x)</td><td>ThermoFisher Scientific</td><td>#11140-035</td><td></td></tr><tr><td>Hematopoietic medium, MyeloCult H5100</td><td>STEMCELL Technologies</td><td>#05150</td><td></td></tr><tr><td>Hexadimethrine bromide (polybrene)</td><td>Sigma-Aldrich</td><td>#H9268</td><td></td></tr><tr><td>Human Dermal Fibroblasts (HDFs)</td><td>ScienCell</td><td>#2320</td><td></td></tr><tr><td>HyClone Dulbecco&#039;s Modified Eagles Medium (DMEM)</td><td>GE Healthcare</td><td>#SH30243.01</td><td></td></tr><tr><td>HyClone Fetal Bovine Serum (FBS)</td><td>GE Healthcare</td><td>#SV30160.03</td><td></td></tr><tr><td>HyClone Penicillin Streptomycin 100x Solution (Pen/Strep)</td><td>GE Healthcare</td><td>#SV30010</td><td></td></tr><tr><td>HyClone Phosphate Buffered Saline solution (PBS)</td><td>GE Healthcare</td><td>#SH30256.01&amp;nbsp;</td><td></td></tr><tr><td>Hydrocortisone</td><td>STEMCELL Technologies</td><td>#7904</td><td></td></tr><tr><td>Mouse serum</td><td>Sigma-Aldrich</td><td>#M5905</td><td></td></tr><tr><td>PE anti-human CD9 Antibody clone HI9a</td><td>BioLegend</td><td>#312105</td><td></td></tr><tr><td>pFUW-tetO-3xFLAG-GATA2</td><td>Addgene</td><td>#125600</td><td></td></tr><tr><td>pFUW-tetO-FOS</td><td>Addgene</td><td>#125598</td><td></td></tr><tr><td>pFUW-tetO-GATA2</td><td>Addgene</td><td>#125028</td><td></td></tr><tr><td>pFUW-tetO-GFI1B</td><td>Addgene</td><td>#125597</td><td></td></tr><tr><td>pLV-tetO-HA-GFI1B</td><td>Addgene</td><td>#125599</td><td></td></tr><tr><td>pMD2.G</td><td>Addgene</td><td>#12259</td><td></td></tr><tr><td>psPAX2</td><td>Addgene</td><td>#12260</td><td></td></tr></tbody></table>

hemogenic reprogramming of human fibroblasts by enforced expression of transcription factors

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

The cellular and molecular mechanisms underlying specification of human hematopoietic stem cells (HSCs) remain elusive. Strategies to recapitulate human HSC emergence in vitro are required to overcome limitations in studying this complex developmental process. Here, we describe a protocol to generate hematopoietic stem and progenitor-like cells from human dermal fibroblasts employing a direct cell reprogramming approach. These cells transit through a hemogenic intermediate cell-type, resembling the endothelial-to-hematopoietic transition (EHT) characteristic of HSC specification. Fibroblasts were reprogrammed to hemogenic cells via transduction with GATA2, GFI1B and FOS transcription factors. This combination of three factors induced morphological changes, expression of hemogenic and hematopoietic markers and dynamic EHT transcriptional programs. Reprogrammed cells generate hematopoietic progeny and repopulate immunodeficient mice for three months. This protocol can be adapted towards the mechanistic dissection of the human EHT process as exemplified here by defining GATA2 targets during the early phases of reprogramming. Thus, human hemogenic reprogramming provides a simple and tractable approach to identify novel markers and regulators of human HSC emergence. In the future, faithful induction of hemogenic fate in fibroblasts may lead to the generation of patient-specific HSCs for transplantation.

A schematic representation of the reprogramming approach using HDFs is illustrated in Figure 1A. Fibroblasts are acquired from commercial sources or collected from human donors and expanded in vitro previous to reprogramming. After plating, cells are transduced twice with GATA2, GFI1B and FOS (and M2rtTA) lentiviruses, and doxycycline is added at day 0 of reprogramming. On day 2, cells are split and plated in hematopoietic medium until day 25 of culture. Reprogrammed cells may be generated at different time points for multiple applications including transplantation in immunocompromised mice, single-cell RNA-sequencing (scRNA-seq) of purified cell populations (day 2 unsorted, day 15 CD49f+ CD34 and day 25 CD49f+CD34+ cells), as well as microscopy and flow cytometry analysis for the cell surface markers CD49f, CD34, CD9 and CD143. Representative cytometry plots show ~17% of reprogrammed cells expressing both CD49f and CD9 (Figure 1B, left panel), after 25 days of reprogramming. The majority of double positive cells express CD143 (~86%), and a small population express CD34 (0.9%), suggesting a dynamic hemogenic fate induction. These markers are not activated in M2rtTA transduced HDFs cultured for 25 days (Figure 1B, right panel). Immunofluorescence images confirm expression of CD9 and CD143 in adherent and round cells, morphologically distinct from fibroblasts that are negative for these markers (Figure 1C). Human hemogenic colonies also express CD49f and CD3414. ScRNA-seq analysis of HDFs, day 2 unsorted cells, and purified reprogrammed cells at day 15 (CD49f+CD34-) and day 25 (CD49f+CD34+) show a stepwise increase in CD49f, CD9 and CD143 expression from day 2 to day 25. CD49f and CD9 positive cells appear first during the reprogramming process, between day 2 and 15, indicating that these molecules may represent markers of early human hemogenesis. CD143 expression starts to be detected at day 15 and CD34 expressing cells are detected only at later time points (day 25). CD34+ umbilical cord blood (UCB) cells were used as reference (Figure 1D).
Figure 2A describes a modified protocol to generate sufficient number of cells for ChIP-seq analysis at the initial stages of hemogenic reprogramming (day 2). First, HDFs are plated at a density two times higher than in the standard protocol (300,000 cells versus 150,000 cells per plate). After transduction, each well is re-plated into a 100 mm dish allowing cells to expand for 6 days before supplementing medium with DOX. Cells are analyzed 2 days after adding DOX and consequent TF expression. Figure 2B shows genome browser profiles of GATA2 binding to genomic regulatory regions of ITGA6 and ACE when cells are co-transduced with the three factors (3TFs) or GATA2 individually. GATA2 also binds to open chromatin regions of CD9 and CD34 genes14.
<img alt="Human cell reprogramming process; FACS analysis; immunofluorescence; gene expression results." class="xfigimg" src="/files/ftp_upload/60112/60112fig1a.jpg" data-altupdatedat="1788282455000" data-alt="Figure 1"> 
Figure 1: Induction of hemogenic fate in human dermal fibroblasts. (A) Experimental strategy for hemogenic reprogramming of human dermal fibroblasts (HDFs). Fibroblasts from skin punch biopsies are collected from donors, expanded and transduced with GATA2, GFI1B, FOS and M2rtTA lentiviruses. Doxycycline (DOX) is added to the culture at day 0 of reprogramming and cells are analyzed at several time points until day 25. scRNA-seq, single cell RNA-sequencing. FACS, Fluorescence-Activated Cell Sorting. (B) Gating strategy used to evaluate the expression of hemogenic/hematopoietic markers by flow cytometry at day 25 after transduction with the three transcription factors (3TFs). Cytometry plots show percentage of double positive cells for CD49f and CD9, gated in the live-cell population (DAPI-negative). Within the double positive population, the expression of CD143 and CD34 is shown. HDFs transduced only with M2rtTA virus under the same culture conditions are used as control. (C) Immunofluorescence images of day 25 reprogrammed colonies confirming the expression of CD9 (upper panel) and CD143 (lower panel). Cells were stained with antibodies (Table of Materials) diluted 1:100 in PBS/2% FBS with mouse serum, incubated 20 min at 37 °C, 5% CO2, washed three times and imaged in PBS/2% FBS. Phase, phase-gradient contrast. Scale bars = 50 µm. (D) ScRNA-seq analysis of 253 cells at different time points. Expression of ITGA6, CD9, ACE and CD34 is activated during reprogramming. Cells are collected at day 2 (unsorted), day 15 (CD49f+CD34-) and day 25 (CD49f+CD34+). HDFs and CD34+ umbilical cord blood (34+UCB) cells are used as references. <a href="https://www.jove.com/files/ftp_upload/60112/60112fig1alarge.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="ChIP-seq workflow diagram; HDF transduction, DOX addition; gene analysis charts for ITGA6, ACE." class="xfigimg" src="/files/ftp_upload/60112/60112fig02.jpg" data-altupdatedat="1788282455000" data-alt="Figure 2"> 
Figure 2: Expansion of human dermal fibroblasts for ChIP-seq analysis. (A) Experimental strategy depicting a modified protocol to generate high numbers of transduced human dermal fibroblasts (HDFs) for ChIP-seq at day 2 of reprogramming. 300,000 cells are plated in 6-well plates and transduced twice with individual factors (pFUW-tetO-FOS, pLV-tetO-HA-GFI1B or pFUW-tetO-3xFLAG-GATA2) or a combination of the three factors (plus M2rtTA). After removing viruses, fibroblasts are expanded for six days in 100 mm dishes. Doxycycline (DOX) is added at day 0 and cells are collected two days after DOX addition. (B) Genome browser profiles highlighting GATA2-binding sites (grey boxes) at ITGA6 and ACE loci two days after transduction with the three transcription factors (3TFs) or with GATA2 alone. The total number of mapped reads is represented on the y-axis. <a href="https://www.jove.com/files/ftp_upload/60112/60112fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Bar graph illustrating percentage of CD49f+CD9+ cells at varying M2rtTA and 3TFs concentrations." src="/files/ftp_upload/60112/60112suppfig1.jpg" data-altupdatedat="1788282455000" data-alt="Supplementary Figure 1"> 
Supplementary Figure 1: Defining an optimized lentiviral volume for efficient hemogenic reprogramming. Increasing volumes of concentrated (10 to 100 μL) pool-produced lentiviral particles (3TFs: GATA2, GFI1B and FOS) are used to transduce human dermal fibroblasts (HDFs), together with M2rtTA at a ratio of 1:1, following steps 4.5−4.12 of the protocol. Reprogrammed cells are analyzed at day 25 to define an optimal volume of transduction for hemogenic reprogramming, given by the percentage of CD49f+CD9+ cells gated in live-cells (DAPI-negative). Cell viability can be assessed by quantifying the absolute number of live cells at day 25. HDFs transduced with M2rtTA (100 μL) are used as negative control. <a href="https://www.jove.com/files/ftp_upload/60112/60112suppfig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Cell growth time-lapse, days -2 to 25; microscopy image showing cellular proliferation stages." src="/files/ftp_upload/60112/60112suppfig2.jpg" data-altupdatedat="1788282455000" data-alt="Supplementary Figure 2"> 
Supplementary Figure 2: Morphology changes during hemogenic reprogramming of human dermal fibroblasts. Human Dermal Fibroblast (HDF) cultures are imaged at the day of the first transduction (day -2), when DOX is added to the cultures (day 0), two days (day 2) and fifteen days (day 15) after DOX supplementation, and at the end-point of the experiment (day 25). Hemogenic colonies at days 15 and 25 are highlighted. Scale bars = 100 µm. <a href="https://www.jove.com/files/ftp_upload/60112/60112suppfig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.

The cellular and molecular mechanisms underlying specification of human hematopoietic stem cells (HSCs) remain elusive. Strategies to recapitulate human ...

hemogenic-reprogramming-human-fibroblasts-enforced-expression

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