Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine

9.3K views

08:31 min

October 11th, 2019

10.3791/60136-v

October 11th, 2019

9.3K views

Yalan Wu^*¹ , Shengjun Wu^*¹ , Xin-an Huang¹ , Qingping Zeng¹ , Tao Deng¹ , Fang Liu¹

¹Institute of Tropical Medicine, Guangzhou University of Chinese Medicine

^* These authors contributed equally

This protocol describes a novel colorimetric method for antimalarial primaquine (PMQ) detection in synthetic urines and human serums.

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Chapters in this video

0:04

Title

1:10

Synthesis of Colored Azos and UV-Vis Measurements

3:01

PMQ Determination and Calibration Curves

5:04

PMQ Extraction from Serum

6:04

Results: Optimization and Calibration of the PMQ Detection Reaction

7:44

Conclusion

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