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Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
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JoVE Journal Medicine
Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine

DOI:
10.3791/60136-v

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08:31 min

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October 11, 2019

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, , , , ,
1Institute of Tropical Medicine,Guangzhou University of Chinese Medicine

Chapters

  • 00:04Title
  • 01:10Synthesis of Colored Azos and UV-Vis Measurements
  • 03:01PMQ Determination and Calibration Curves
  • 05:04PMQ Extraction from Serum
  • 06:04Results: Optimization and Calibration of the PMQ Detection Reaction
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a novel colorimetric method for antimalarial primaquine (PMQ) detection in synthetic urines and human serums.

Tags

Keywords: Griess ReactionUV-VisNaked-eye DetectionAnti-malarialPrimaquineHPLCAnilineSodium NitrateAzo ProductFlash ChromatographyLyophilizationAbsorption Spectra4-methoxyanilineHydrogen Chloride

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