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Developmental Biology

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JoVE Journal Developmental Biology
Isolering af specifikke neuron populationer fra rundorm Caenorhabditis elegans
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Isolering af specifikke neuron populationer fra rundorm Caenorhabditis elegans

Article DOI: 10.3791/60145-v • 09:42 min • August 6th, 2019
August 6th, 2019 •
1, 1, 1,2,3,4
1Department of Biochemistry, University of Nebraska, 2Nebraska Redox Biology Center, University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, 4Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

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Her præsenterer vi en protokol for enkel isolering af specifikke grupper af levende neuronal celler, der udtrykker grønt fluorescerende protein fra transgene Caenorhabditis elegans linjer. Denne metode muliggør en række ex vivo-undersøgelser med fokus på specifikke neuroner og har kapacitet til at isolere celler til yderligere kort tids dyrkning.

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Udviklingsmæssige biologi C. elegans celle isolation neuroner aldring neuronal markører grøn fluorescerende protein
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