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Journal / Genetics / Analyzing Satellite Cell Function During Skeletal …
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Genetics

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JoVE Journal Genetics
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Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Article DOI: 10.3791/60194-v • 06:37 min • September 18th, 2019
September 18th, 2019 •
*1, *1, 1, 1, 1, 1
1Leibniz-Institute on Aging - Fritz-Lipmann-Institute
* These authors contributed equally

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Summary
Automatic Translation

We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.

Tags

Satellite Cell Function Skeletal Muscle Regeneration Cardiotoxin Injury Self-delivering SiRNA Intramuscular Injection Analysis Of Muscle Regeneration Functional Loss Of Single Genes Transgenic Animals Toe Pinch Response Injection Area Disinfection Insulin Syringe 29-gauge Needle Cardiotoxin Injection Tibialis Anterior Muscle Injury Recumbency Monitoring
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