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Optical Imaging of Isolated Murine Ventricular Myocytes
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optical Imaging of Isolated Murine Ventricular Myocytes

Optical Imaging of Isolated Murine Ventricular Myocytes

DOI:
10.3791/60196-v

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11:32 min

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January 17, 2020

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, , , , , , ,
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center

Chapters

  • 00:05Title
  • 01:26Preparation of Coverslips
  • 02:08Myocyte Isolation
  • 05:53Membrane Potential Dye Loading
  • 07:01Photometry and Charge Coupled Device Recordings
  • 09:21Results: Calcium and Sarcomere Shortening
  • 10:48Conclusion

Summary

Automatic Translation

Automatic Translation

We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.

Tags

Keywords: Optical ImagingMurine Ventricular MyocytesHeart FailureCardiac MetabolismExcitation-contraction CouplingCardiac Myocyte IsolationFluorescent ProbeCardiac ToxicityHeart Failure TherapeuticsTransgenic MiceLamininLangendorf PerfusionKHB-HB SolutionDigestion Buffer

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