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Journal / Bioengineering / Imaging ottico di miociti Murine Ventricolari…
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Bioengineering

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JoVE Journal Bioengineering
Imaging ottico di miociti Murine Ventricolari isolati
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Imaging ottico di miociti Murine Ventricolari isolati

Article DOI: 10.3791/60196-v • 11:32 min • January 17th, 2020
January 17th, 2020 •
1,2, 3, 3, 3, 3, 1, 3, 3
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center

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Presentiamo la metodologia per l'isolamento dei miociti murini e come ottenere tracce di tensione o calcio contemporaneamente con tracce di accorciamento sarcomero utilizzando la fotometria a fluorescenza con misurazioni simultanee della geometria cellulare digitale.

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Retrazione Problema 155 miocito isolamento enzimatico potenziale di azione ottica calcio accoppiamento eccitazione-contrazione durata potenziale azione
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