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September 16, 2019
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Summary
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A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.
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Cite this Article
Marek, A., Schüler, C., Satué, M., Haigl, B., Erben, R. G. A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones. J. Vis. Exp. (151), e60197, doi:10.3791/60197 (2019).
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