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Biology

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A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
 

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Article DOI: 10.3791/60197-v 10:11 min September 16th, 2019
September 16th, 2019

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Summary

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

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Keywords: Laser Capture Microdissection RNA Extraction Mouse Bone Cryosections Gene Expression Analysis Tissue Sectioning OCT Compound Cryostat
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