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JoVE Journal Biology
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
 

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Article doi: 10.3791/60197
September 16th, 2019 • Usage Statistics
1, 1, 1, 1, 1
1Department of Biomedical Research, University of Veterinary Medicine Vienna

Summary
Automatic Translation
September 16th, 2019

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

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