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A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
JoVE Journal
Biology
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JoVE Journal Biology
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

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10:11 min

September 16, 2019

DOI:

10:11 min
September 16, 2019

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Summary

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A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

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