Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

DOI:

10.3791/60235-v

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07:16 min

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September 27, 2019

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Yixuan Sun, Peipei Zhou, Qianhui Zhao, Zhefeng Gong

¹Department of Neurobiology,Zhejiang University

Chapters

00:04Title
00:27Agar Plate Preparation and Visual Stimulation System Setup
02:04Imaging System Setup
03:58Light Avoidance Behavior Recording
05:48Results: Representative Larva Reactions to Light Spot Entrance
06:24Conclusion

Summary

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This protocol introduces a light-spot assay to investigate Drosophila larval phototactic behavior. In this assay, a light spot is generated as light stimulation, and the process of larval light avoidance is recorded by an infrared light-based imaging system.

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

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