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High Throughput Co-culture Assays for the Investigation of Microbial Interactions
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Chapters
- 00:04Title
- 01:06Preparation of Overnight Cultures and Bioassay Plates
- 02:20Inoculating Bioassay Plates with the Test Organism
- 02:58Preparation and Inoculation of the Target Organism
- 05:02Results: Co-culture Interaction Assays Uncover Siderophore-mediated Inhibition of Coagulase-negative staphylococci by Corynebacterium propinquum
- 06:06Conclusion
The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.
