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December 19, 2019
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Summary
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マウス前脳の組織図スライス培養における遺伝的にコードされたFRETベースのセンサATeam1.03YEMKの細胞型特異的発現に関するプロトコルについて説明する。さらに、ニューロンおよびアストロサイトにおける細胞ATPレベルの動的イメージングにこのセンサを使用する方法を示す。
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Cite this Article
Lerchundi, R., Kafitz, K. W., Färfers, M., Beyer, F., Huang, N., Rose, C. R. Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK. J. Vis. Exp. (154), e60294, doi:10.3791/60294 (2019).
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