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JoVE Journal
Immunology and Infection

This content is Open Access.

Imaging the Human Immunological Synapse
 

Imaging the Human Immunological Synapse

Article doi: 10.3791/60312
December 26th, 2019

Summary December 26th, 2019

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).

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