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JoVE Journal
Immunology and Infection

This content is Open Access.

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Article DOI: 10.3791/60312-v
December 26th, 2019


Summary December 26th, 2019

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).


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