Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Imaging the Human Immunological Synapse
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Imaging the Human Immunological Synapse
Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
Journal (Immunology and Infection)
Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth
Journal (Immunology and Infection)
Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth
 

Imaging the Human Immunological Synapse

Article DOI: 10.3791/60312-v • 09:37 min • December 26th, 2019
December 26th, 2019 •
*1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 1,2, *1,2
1Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, 2Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, 3Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).

Tags

Immunological Synapse Cell-to-cell Conjugation Antigen-presenting Cell T-helper Lymphocyte Immune Synapse Formation Trafficking Events Polarized Secretion Wide-field Fluorescence Microscopy Image Processing Signal-to-noise Ratio Temporal Resolution Fluorochromes Fluorescence Bleaching End Point Cell Fixation Protocols Immunofluorescence Staining Laser Scanning Fluorescent Microscopy Fibronectin Microwell Chamber Slide PVS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter