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Immunology and Infection

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JoVE Journal Immunology and Infection
Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models
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Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

Article DOI: 10.3791/60315-v • 09:54 min • December 3rd, 2019
December 3rd, 2019 •
1,2, 1, 1, 1, 1, 1
1Institute of Human Virology, School of Medicine, University of Maryland, 2Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia

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Summary
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Described here are three experimental approaches for studying the dynamics of HIV infection in humanized mice. The first permits the study of chronic infection events, whereas the two latter allows for the study of acute events after primary infection or viral reactivation.

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Chronic Acute Reactivated HIV Infection Humanized Immunodeficient Mouse Models NSG Humanized Mice Dynamics Of HIV Infection Pre-clinical Settings Cell Preparation Procedure Sandra Medina-Moreno Laboratory Research Supervisor Disposable Personal Protective Equipment Sterile Scrubs Gloves Dedicated Shoes Shoe Covers Mask Goggles Hair And Beard Bonnets Sterile Lab Coat Chronic Model Frozen CD34+human Stem Cells RPMI 1640 Media 10%FBS Certified Biosafety Cabinet Sterile Conditions Trypan Blue Exclusion Staining Hemocytometer Centrifuge Cold 1X PBS Injection
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