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Prostate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
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Cancer Research
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JoVE Journal Cancer Research
Prostate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses

Prostate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses

DOI:
10.3791/60346-v

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08:36 min

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October 24, 2019

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, , ,
1Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 2Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 01:17Isolation, Enzymatic Digestion, and Establishments of Organoids
  • 03:06Assessing Organoid Formation Capacity
  • 04:05Determining Pharmacological Responses of Organoids
  • 05:44Results: Organoid Seeding Efficiency and Pharmacological Response
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to study pharmacological responses in prostate epithelial organoids. Organoids closely resemble in vivo biology and recapitulate patient genetics, making them attractive model systems. Prostate organoids can be established from wildtype prostates, genetically engineered mouse models, benign human tissue, and advanced prostate cancer.

Tags

Keywords: Prostate Organoid CulturesPharmacological ResponsesGenotypeMutational ProfilesIn Vitro SystemThree-dimensional StructureBasement Membrane MatrixDrug ResistanceDome Plating MethodSingle Cell SuspensionCell CountingDownstream ApplicationsTrypsin ReplacementCell Dissociation

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