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Immunology and Infection
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Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

DOI:
10.3791/60350-v

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06:33 min

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October 29, 2019

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, ,
1Department of Microbiology and Molecular Genetics,Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

Chapters

  • 00:04Title
  • 00:22Slide Preparation
  • 01:41Imaging
  • 03:05Cell Reconstruction
  • 05:10Results: Representative Assessment of Protein Localization to Specific Cellular Geometries
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol explains how to prepare and mount bacterial samples for live three-dimensional imaging and how to reconstruct the three-dimensional shape of E. coli from those images.

Tags

Keywords: 3D ImagingBacterial CellsCellular RepresentationProtein LocalizationMicroscopyAgarose PadsE. ColiZ-stackFluorescence Imaging

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