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JoVE Journal
Immunology and Infection

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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
 

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Article DOI: 10.3791/60359-v
October 31st, 2019

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Summary October 31st, 2019

Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.

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