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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

DOI:
10.3791/60359-v

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08:27 min

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October 31, 2019

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1Public Health Research Institute, New Jersey Medical School,Rutgers, The State University of New Jersey

Chapters

  • 00:04Title
  • 00:47Preparation of Iron-depleted Defined Medium
  • 01:58Growing Mycobacteria in Iron-limited Conditions
  • 03:44Collection, Isolation and Quantification of MEVs (Mycobacterial Extracellular Vesicles)
  • 06:38Results: MEV Purification and Quantification
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.

Tags

Keywords: Extracellular VesiclesMycobacteriaIron-limitedMembrane VesiclesMycobacterium TuberculosisMycobacterium SmegmatisMinimal MediumMetal Chelating ResinBacterial CultureAgar PlatesSpectrophotometryLate Logarithmic Phase

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