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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Chapters
- 00:01Title
- 01:13Waveguide Cleaning
- 02:05Sample Chamber Preparation
- 02:41Fluorescent Labelling
- 04:12Setup Components
- 04:56Waveguide Coupling
- 07:26Diffraction Limited Imaging
- 08:42dSTORM Imaging
- 09:35dSTORM Image Reconstruction
- 10:42Results
- 12:27Conclusion
Chip-based super-resolution optical microscopy is a novel approach to fluorescence microscopy and offers advantages in cost effectiveness and throughput. Here, the protocols for chip preparation and imaging are shown for TIRF microscopy and localization-based super-resolution microscopy.
