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Generation of Multicellular Human Primary Endometrial Organoids
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Multicellular Human Primary Endometrial Organoids

Generation of Multicellular Human Primary Endometrial Organoids

DOI:
10.3791/60384-v

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09:20 min

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October 04, 2019

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, , , ,
1Department of Obstetrics and Gynecology,Northwestern University, 2National Center for Genetic Engineering and Biotechnology (BIOTEC),National Science and Technology Development Agency (NSTDA)

Chapters

  • 00:04Title
  • 01:39Preparation of Agarose Molds and Generation of Endometrial Organoids
  • 04:58Harvesting Endometrial Organoids for Experiments
  • 06:40Results: Analysis of Generated Multicellular Human Primary Endometrial Organoids
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to generate human primary endometrial organoids that consist of epithelial and stromal cells and retain characteristics of the native endometrial tissue is presented. This protocol describes methods from uterine tissue acquisition to the histologic processing of endometrial organoids.

Tags

Keywords: EndometriumOrganoids3D StructureEpithelial CellsStromal CellsHormone ImbalanceCancerParacrine ActionsCell IsolationEnzyme SolutionCell Strainer

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